| Schematic representation of the Plasmodium vivax circumsporozoite protein (CSP) recombinant antigens. The individual recombinant proteins have the specific ...FigUre1-uploadedbyChiaraAndolinaContentmaybesubjecttocopyright.DownloadViewpublicationCopyreferenceCopycaptionEmbedfigure|SchematicrepresentationofthePlasmodiumvivaxcircumsporozoiteprotein(CSP)recombinantantigens.Theindividualrecombinantproteinshavethespecificindividualrepeats[yPvCSP-VK210(n=19),yPvCSP-VK247(n=19),oryPvCSP-P.vivax-like(n=16)]andtheC-terminal(CT)region.Foreachrepeatsequence,theaminoacidspositionsandthehistidinetagareindicated.TheclassicrepeatsequenceVK210isrepresentedinyellow,VK247ingreen,andP.vivax-likeinblue.TheyPvCSP-Allepitopesmergestherepeatsofallthreeallelicvariantsdescribeabove(n=6/6/5repeatsequencesofVK210/P.vivax-like/VK247,respectively)andtheCTregion. Sourcepublication+1VaccineContainingtheThreeAllelicVariantsofthePlasmodiumvivaxCircumsporozoiteAntigenInducesProtectioninMiceafterChallengewithaTransgenicRodentMalariaParasiteArticleFull-textavailableOct2017AlbaMarinaGimenezLucianaLimaKatiaSanchesFrançoso[...]IreneSoaresPlasmodiumvivaxisthemostcommonspeciesthatcausemalariaoutsideoftheAfricancontinent.Thedevelopmentofanefficaciousvaccinewouldcontributegreatlytocontrolmalaria.Recently,usingbacterialandadenoviralrecombinantproteinsbasedontheP.vivaxcircumsporozoiteprotein(CSP),wedemonstratedthepossibilityofelicitingstron...ContextsinsourcepublicationContext1...andyPvCSP-AllepitopesweresynthetizedbyGenScriptUSA,Inc.(Piscataway,NJ,USA)usingcodonoptimizationtoimproveexpressioninP.pastoris.Figure1showsaschematicrepresentationofthese2http://www.mctic.gov.br/mctic/opencms/textogeral/concea.html.recombinantproteins....Viewinfull-textContext2...least50,000cellswereacquiredonaBDFACSCantoIIflowcytometerandthenanalyzedwithFlowJo8.7software.GatingstrategyisdepictedinFigureS1inSupplementaryMaterial....Viewinfull-textSimilarpublicationsNaturallyAcquiredAntibodyResponsetoMalariaTransmissionBlockingVaccin