Download scientific diagram | Life cycle of Plasmodium. falciparum. Schematic illustration of the life cycle of P. falciparum. The blood stages on which ...Figure1-uploadedbyMasahiroEnomotoContentmaybesubjecttocopyright.DownloadViewpublicationCopyreferenceCopycaptionEmbedfigureLifecycleofPlasmodium.falciparum.SchematicillustrationofthelifecycleofP.falciparum.Thebloodstagesonwhichthisstudyisfocusedareshownindetail.doi:10.1371/journal.pone.0039499.g001Sourcepublication+3BlockageofSpontaneousCa2+OscillationCausesCellDeathinIntraerythrociticPlasmodiumfalciparumArticleFull-textavailableJul2012MasahiroEnomotoShin-ichiroKawazuSatoruKawai[...]KatsuhikoMikoshibaMalariaremainsoneoftheworld'smostimportantinfectiousdiseasesandisresponsibleforenormousmortalityandmorbidity.Resistancetoantimalarialdrugsisachallengingprobleminmalariacontrol.ClinicalmalariaisassociatedwiththeproliferationanddevelopmentofPlasmodiumparasitesinhumanerythrocytes.Especially,thedevelopment...ContextsinsourcepublicationContext1...continuestobeaworldwidepublichealthproblemcausingsignificantmorbidityandmortalityanditsresistancetoexistingantimalarialdrugsisagrowingproblem[1].ThelifecycleofPlasmodiumspeciesiscomplex(Fig.1).Infectionofhumansbeginswithasmallinoculumofsporozoitesfromthesalivaryglandsofablood-feedingAnophelesmosquito.Sporozoitespene-tratelivercells,transformandmultiplyasexuallytoproducethousandsoffreemerozoites(liverstage).Eachoftheseasexualmerozoitesinvadesanerythrocyteandentersintoanother...Viewinfull-textContext2...inP.falciparumhasbeendemonstrated[23].Treatmentwith100mM2-APBalmostcompletelyblockedCa2+oscillations(Fig.2AandB,right).Ontheotherhand,inthelateringforms(LRf),latetrophozoites(LT),schizonts(S)andmerozoites(M),smallperiodicCa2+fluctuationswereobserved,andnotableeffectsof2-APBwerenotdetected(Fig.S1).Toinvestigatetheeffectsof2-APBindetail,weperformedquantitativeanalysisoftheeffectof2-APBontheamplitudeofperiodicCa2+fluctuationsinlateringform,latetrophozoite,schizontandmerozoitestages.Them