Final Report Summary - LASSA VIRUS RECEPTOR (The interaction of Lassa virus with its cellular receptor alpha-dystroglycan). Background and project ...CORDISBringingyoutheresultsofEUresearchandinnovationframeworkprogrammessince1990:Horizon2020,FP7andearlierHOMERESULTSPACKSPROJECTS&RESULTSRESEARCH*EUMAGAZINESNEWS&EVENTSABOUTUSAnerroroccurredWearesorry...anunexpectederroroccurredduringexecution.Followuson:AboutCORDISCookiesLegalNoticeContactManagedbytheEUPublicationsOffice

LASSA VIRUS SHOWS COMPLEX RECEPTOR USE. The first LASV receptor was identified as dystroglycan (DG), a ubiquitously expressed conserved cellular ...SkiptomaincontentGemLassaVirusCellEntryRevealsNewAspectsofVirus-HostCellInteractionGiuliaTorriani,ClaraGalan-Navarro,StefanKunzBillyTsai,EditorGiuliaTorrianiFindthisauthoronGoogleScholarFindthisauthoronPubMedSearchforthisauthoronthissiteClaraGalan-NavarroFindthisauthoronGoogleScholarFindthisauthoronPubMedSearchforthisauthoronthissiteStefanKunzFindthisauthoronGoogleScholarFindthisauthoronPubMedSearchforthisauthoronthissiteBillyTsaiUniversityofMichiganMedicalSchoolRoles:EditorFindthisauthoronGoogleScholarFindthisauthoronPubMedSearchforthisauthoronthissiteDOI: 10.1128/JVI.01902-16ArticleFigures&DataInfo&MetricsPDFABSTRACTViralentryrepresentsthefirststepofeveryviralinfectionandisadeterminantforthehostrangeanddiseasepotentialofavirus.Here,wereviewthelatestdevelopmentsoncellentryofthehighlypathogenicOldWorldarenavirusLassavirus,providingnovelinsightsintothecomplexvirus-hostcellinteractionofthisimportanthumanpathogen.Wewillcovernewdiscoveriesonthemolecularmechanismsofreceptorrecognition,endocytosis,andtheuseoflateendosomalentryfactors.INTRODUCTIONAmongthearenaviruses,Lassavirus(LASV)representsthemostprevalenthumanpathogen,withseveralhundredthousandinfectionsperyear(1).CarriedbypersistentinfectionofthereservoirrodenthostMastomysnatalensis,LASVisendemicinlargepartsofwesternAfrica.Arenavirusesareenvelopednegative-strandRNAviruseswithanonlyticlifecycleconfinedtothecytosol.Thearenavirusgenomecomprisesasmall(S)RNAsegmentencodingtheenvelopeglycoproteinprecursor(GPC)andthenucleoprotein(NP)andanLsegmentcodingforthematrixprotein(Z)aswellastheviralpolymerase(L)(2).TheGPCissynthesizedasasinglepolypeptideandundergoesprocessing,yieldingastablesignalpeptide(SSP),N-terminalGP1,andtransmembraneGP2.GP1bindstocellularreceptors,whereasGP2mediatesviralfusionandstructurallyresemblesclassIviralfusionproteins.Uponreceptorbinding,LASVentersthehostcellv

FP7,LASSA VIRUS RECEPTOR,PEOPLE-2007-4-3.IRG,CENTRE HOSPITALIER UNIVERSITAIRE VAUDOIS(CH)CORDISBringingyoutheresultsofEUresearchandinnovationframeworkprogrammessince1990:Horizon2020,FP7andearlierHOMERESULTSPACKSPROJECTS&RESULTSRESEARCH*EUMAGAZINESNEWS&EVENTSABOUTUSAnerroroccurredWearesorry...anunexpectederroroccurredduringexecution.Followuson:AboutCORDISCookiesLegalNoticeContactManagedbytheEUPublicationsOffice

We found that Lassa virus readily engaged its cell-surface receptor α-dystroglycan in avian cells, but virus entry in susceptible species involved a ...SkiptomaincontentReadourCOVID-19researchandnews.shareScience 27Jun2014:Vol.344,Issue6191,pp.1506-1510DOI:10.1126/science.1252480LucasT.Jae1NetherlandsCancerInstitute,Plesmanlaan121,1066CX,Amsterdam,Netherlands.FindthisauthoronGoogleScholarFindthisauthoronPubMedSearchforthisauthoronthissiteMatthijsRaaben1NetherlandsCancerInstitute,Plesmanlaan121,1066CX,Amsterdam,Netherlands.2DepartmentofMicrobiologyandImmunobiology,HarvardMedicalSchool,77AvenueLouisPasteur,Boston,MA02115,USA.FindthisauthoronGoogleScholarFindthisauthoronPubMedSearchforthisauthoronthissiteAndrewS.Herbert3U.S.ArmyMedicalResearchInstituteofInfectiousDiseases,1425PorterStreet,FortDetrick,MD21702–5011,USA.FindthisauthoronGoogleScholarFindthisauthoronPubMedSearchforthisauthoronthissiteAnaI.Kuehne3U.S.ArmyMedicalResearchInstituteofInfectiousDiseases,1425PorterStreet,FortDetrick,MD21702–5011,USA.FindthisauthoronGoogleScholarFindthisauthoronPubMedSearchforthisauthoronthissiteArielS.Wirchnianski3U.S.ArmyMedicalResearchInstituteofInfectiousDiseases,1425PorterStreet,FortDetrick,MD21702–5011,USA.FindthisauthoronGoogleScholarFindthisauthoronPubMedSearchforthisauthoronthissiteTimothyK.Soh2DepartmentofMicrobiologyandImmunobiology,HarvardMedicalSchool,77AvenueLouisPasteur,Boston,MA02115,USA.FindthisauthoronGoogleScholarFindthisauthoronPubMedSearchforthisauthoronthissiteSarahH.Stubbs2DepartmentofMicrobiologyandImmunobiology,HarvardMedicalSchool,77AvenueLouisPasteur,Boston,MA02115,USA.FindthisauthoronGoogleScholarFindthisauthoronPubMedSearchforthisauthoronthissiteHansJanssen1NetherlandsCancerInstitute,Plesmanlaan121,1066CX,Amsterdam,Netherlands.FindthisauthoronGoogleScholarFindthisauthoronPubMedSearchforthisauthoronthissiteMarkusDamme4BiochemischesInstitut,ChristianAlbrechts-UniversitätKiel,24118Kiel,Germany.FindthisauthoronGoogleScholarFindthisauthoronPubMedSearchforthi

Abstract. The cellular receptor for the Old World arenaviruses Lassa fever virus (LFV) and lymphocytic choriomeningitis virus (LCMV) has recently been identified ...NCBISkiptomaincontentSkiptonavigationResourcesHowToAboutNCBIAccesskeysMyNCBISignintoNCBISignOutJournalListJVirolv.79(10);2005MayPMC1091707JVirol.2005May;79(10):5979–5987.doi: 10.1128/JVI.79.10.5979-5987.2005PMCID:PMC1091707PMID:15857984CharacterizationoftheInteractionofLassaFeverViruswithItsCellularReceptorα-DystroglycanStefanKunz,1,*JillianM.Rojek,1MarPerez,1ChristinaF.Spiropoulou,2andMichaelB.A.Oldstone1StefanKunzDivisionofVirology,DepartmentofNeuropharmacology,TheScrippsResearchInstitute,LaJolla,California92037,1SpecialPathogensBranch,CentersforDiseaseControlandPrevention,Atlanta,Georgia303332FindarticlesbyStefanKunzJillianM.RojekDivisionofVirology,DepartmentofNeuropharmacology,TheScrippsResearchInstitute,LaJolla,California92037,1SpecialPathogensBranch,CentersforDiseaseControlandPrevention,Atlanta,Georgia303332FindarticlesbyJillianM.RojekMarPerezDivisionofVirology,DepartmentofNeuropharmacology,TheScrippsResearchInstitute,LaJolla,California92037,1SpecialPathogensBranch,CentersforDiseaseControlandPrevention,Atlanta,Georgia303332FindarticlesbyMarPerezChristinaF.SpiropoulouDivisionofVirology,DepartmentofNeuropharmacology,TheScrippsResearchInstitute,LaJolla,California92037,1SpecialPathogensBranch,CentersforDiseaseControlandPrevention,Atlanta,Georgia303332FindarticlesbyChristinaF.SpiropoulouMichaelB.A.OldstoneDivisionofVirology,DepartmentofNeuropharmacology,TheScrippsResearchInstitute,LaJolla,California92037,1SpecialPathogensBranch,CentersforDiseaseControlandPrevention,Atlanta,Georgia303332FindarticlesbyMichaelB.A.OldstoneAuthorinformationArticlenotesCopyrightandLicenseinformationDisclaimerDivisionofVirology,DepartmentofNeuropharmacology,TheScrippsResearchInstitute,LaJolla,California92037,1SpecialPathogensBranch,CentersforDiseaseControlandPrevention,Atlanta,Georgia303332*Correspondingauthor.Mailingaddress:D

Ebola virus and Lassa virus belong to different virus families that can cause viral hemorrhagic fever, a life- threatening disease in humans with limited treatment.ThissiterequiresCookiestobeenabledtofunction.PleaseensureCookiesareturnedonandthenre-visitthedesiredpage.

The pathogenic Old World arenavirus Lassa virus (LASV) causes a severe hemorrhagic fever with a high rate of mortality in humans. Several LASV receptors ...SkiptomaincontentVirus-CellInteractionsLassaVirusCellEntryviaDystroglycanInvolvesanUnusualPathwayofMacropinocytosisJoelOppliger,GiuliaTorriani,AntonioHerrador,StefanKunzT.S.Dermody,EditorJoelOppligerFindthisauthoronGoogleScholarFindthisauthoronPubMedSearchforthisauthoronthissiteGiuliaTorrianiFindthisauthoronGoogleScholarFindthisauthoronPubMedSearchforthisauthoronthissiteAntonioHerradorFindthisauthoronGoogleScholarFindthisauthoronPubMedSearchforthisauthoronthissiteStefanKunzFindthisauthoronGoogleScholarFindthisauthoronPubMedSearchforthisauthoronthissiteT.S.DermodyUniversityofPittsburghSchoolofMedicineRoles:EditorFindthisauthoronGoogleScholarFindthisauthoronPubMedSearchforthisauthoronthissiteDOI: 10.1128/JVI.00257-16ArticleFigures&DataInfo&MetricsPDFABSTRACTThepathogenicOldWorldarenavirusLassavirus(LASV)causesaseverehemorrhagicfeverwithahighrateofmortalityinhumans.SeveralLASVreceptors,includingdystroglycan(DG),TAMreceptortyrosinekinases,andC-typelectins,havebeenidentified,suggestingcomplexreceptoruse.Uponreceptorbinding,LASVentersthehostcellviaanunknownclathrin-anddynamin-independentpathwaythatdeliversthevirustolateendosomes,wherefusionoccurs.HereweinvestigatedthemechanismsunderlyingLASVendocytosisinhumancellsinthecontextofproductivearenavirusinfection,usingrecombinantlymphocyticchoriomeningitisvirus(rLCMV)expressingtheLASVglycoprotein(rLCMV-LASVGP).WefoundthatrLCMV-LASVGPenteredhumanepithelialcellsviaDGusingamacropinocytosis-relatedpathwayindependentlyofalternativereceptors.Dystroglycan-mediatedentryofrLCMV-LASVGPrequiredsodiumhydrogenexchangers,actin,andtheGTPaseCdc42anditsdownstreamtargets,p21-activatingkinase-1(PAK1)andWiskott-Aldrichsyndromeprotein(N-Wasp).Unlikeothervirusesthatentercellsviamacropinocytosis,rLCMV-LASVGPentrydidnotinduceovertchangesincellularmorphologyandhardlyaffectedactindynamicsorfluiduptake

NCBISkiptomaincontentSkiptonavigationResourcesHowToAboutNCBIAccesskeysMyNCBISignintoNCBISignOutJournalListElsevierPublicHealthEmergencyCollectionPMC6083867JMolBiol.2018Aug17;430(17):2590–2611.Publishedonline2018Jun18.doi: 10.1016/j.jmb.2018.06.024PMCID:PMC6083867NIHMSID:NIHMS976031PMID:29924965Virus–ReceptorInteractions:TheKeytoCellularInvasionMelissaS.Maginnis⁎AuthorinformationArticlenotesCopyrightandLicenseinformationDisclaimerDepartmentofMolecularandBiomedicalSciences,TheUniversityofMaine,Orono,ME04469-5735,USA⁎DepartmentofMolecularandBiomedicalSciences,UniversityofMaine,5735HitchnerHallRoom326,Orono,ME04469-5735,USA.Received2018Mar25;Revised2018Jun4;Accepted2018Jun8.Copyright©2018ElsevierLtd.Allrightsreserved.SinceJanuary2020ElsevierhascreatedaCOVID-19resourcecentrewithfreeinformationinEnglishandMandarinonthenovelcoronavirusCOVID-19.TheCOVID-19resourcecentreishostedonElsevierConnect,thecompany'spublicnewsandinformationwebsite.ElsevierherebygrantspermissiontomakeallitsCOVID-19-relatedresearchthatisavailableontheCOVID-19resourcecentre-includingthisresearchcontent-immediatelyavailableinPubMedCentralandotherpubliclyfundedrepositories,suchastheWHOCOVIDdatabasewithrightsforunrestrictedresearchre-useandanalysesinanyformorbyanymeanswithacknowledgementoftheoriginalsource.ThesepermissionsaregrantedforfreebyElsevierforaslongastheCOVID-19resourcecentreremainsactive.ThisarticlehasbeencitedbyotherarticlesinPMC.Virus–receptorinteractionsplayakeyregulatoryroleinviralhostrange,tissuetropism,andviralpathogenesis.Virusesutilizeelegantstrategiestoattachtooneormultiplereceptors,overcometheplasmamembranebarrier,enter,andaccessthenecessaryhostcellmachinery.Theviralattachmentproteincanbeviewedasthe“key”thatunlockshostcellsbyinteractingwiththe“lock”—thereceptor—onthecellsurface,andtheselock-and-keyinteractionsarecriticalforvirusestosuccessfullyinvadehostcells.Manycommonthemeshaveemergedinvirus–receptorutilizationwithinandacrossvirusfamiliesdemonstratingthatvirusesoftentargetparticularcl

The Lassa virus particle is round, oval, or pleomorphic, 110 to 130 nm in diameter, and enveloped. Its genome consists of two single-stranded RNA segment – the large L segment and the small S segment.SkiptomaincontentCloseSearchMenuLASSAEBOLALassaLassafever(Lassa)isanacuteviralhemorrhagicfeverendemictoWestAfrica.Sinceitsdiscoveryin1969inthevillageofLassainBornoState,Nigeria,therehavebeencountlessoutbreaksofvariousmagnitudeandseverityacrossWestAfrica.Itisestimatedthatmanythousandsofpeopleareinfectedwiththevirusannually,withthousandsofdeaths.Casefatalityratesininfectedpatientscanreachmorethan80%.Thesenumbers,however,areonlyestimates,andinrealitylittleisknownaboutthetruemorbidityandmortalityfromthisdisease.GeographicalDistributionLassaisendemictoWestAfrica.ConfirmedincidenceshavebeenrecordedinSierraLeone,Liberia,Guinea,Nigeria,IvoryCoast,Togo,andMali.However,concernsexistthattheremaybeLassa(andLassa-like)virusesinothercountriessuchasCentralAfricanRepublic,BurkinaFaso,Ghana,Benin,andCameroon–someofwhichhavehadsporadiccases.Furthermore,MastomysrodentsaredistributedacrosstheAfricancontinent,indicatingastrongpossibilityforthespreadofthediseases,includingLassa,thattheycarry.InfectionswithLassavirusHumanscontractthevirusprimarilythroughcontactwiththecontaminatedexcretaofMastomysnatalensisrodents(commonlyknownastheMultimammaterat),whichisthenaturalreservoirforthevirus.Littleisknownregardingthetransmissionofthevirusfromtherodentreservoirtothehumanhost,althoughthereiscompellingevidencethatArenavirusesarestableandinfectiousbytheaerosolrouteinnonhumanprimates.Therodentsliveinhouseswithhumansanddepositexcretaonfloors,tables,bedsandfood.Consequentlythevirusistransmittedtohumansthroughcutsandscratches,orinhaledviadustparticlesintheair.InsomeregionsMastomysrodentsarealsoconsumedasfood.Secondaryhuman-to-humantransmissionscanalsooccur,althoughsuchscenariosaremuchrarerthanrodent-causedinfections.Historically,small-scaleoutbreakscausedbyhuman-to-humantransmissionshavetakenplace,butmostly