Here, we present an accurate sex prediction model to discriminate fetal sex in both dichorionic diamniotic (DCDA) and monochorionic ...ArticlePDFAvailableFetalsexdeterminationintwinpregnanciesusingnon-invasiveprenataltestingJuly2019npjGenomicMedicine4(1)DOI:10.1038/s41525-019-0089-4Authors:DarineVillelaUniversityofSãoPauloDownloadfull-textPDFReadfull-textDownloadfull-textPDFReadfull-textDownloadcitationCopylinkLinkcopiedReadfull-textDownloadcitationCopylinkLinkcopiedCitations(3)References(28)Figures(2)AbstractandFiguresNon-invasiveprenataltesting(NIPT)isaccurateforfetalsexdeterminationinsingletonpregnancies,butitsaccuracyisnotwellestablishedintwinpregnancies.Here,wepresentanaccuratesexpredictionmodeltodiscriminatefetalsexinbothdichorionicdiamniotic(DCDA)andmonochorionicdiamniotic/monochorionicmonoamniotic(MCDA/MCMA)twinpregnancies.Aretrospectiveanalysiswasperformedusingatotalof198twinpregnancieswithdocumentedsex.ThepredictionwasbasedonamultinomiallogisticregressionusingthenormalizedfrequencyofXandYchromosomes,andfetalfractionestimation.Asecond-stepregressionanalysiswasappliedwhenoneorbothtwinswerepredictedtobemale.Themodeldeterminesfetalsexwith100%sensitivityandspecificitywhenbothtwinsarefemale,andwith98%sensitivityand95%specificitywhenamaleispresent.Sincesexdeterminationcanbeclinicallyimportant,implementingfetalsexdeterminationintwinswillimproveoveralltwinpregnanciesmanagement.StatisticalparametersofNIPTsequencingdata… TwincharacteristicsDCDA(n=112)MCDA/MCMA(n=86)… Figures-uploadedbyDarineVillelaAuthorcontentAllfigurecontentinthisareawasuploadedbyDarineVillelaContentmaybesubjecttocopyright.Discovertheworld'sresearch20+millionmembers135+millionpublications700k+researchprojectsJoinforfreePublicFull-text1ContentuploadedbyDarineVillelaAuthorcontentAllcontentinthisareawasuploadedbyDarineVillelaonJul04,2019Contentmaybesubjecttocopyright.ARTICLEOPENFetalsexdeterminationintwinpregnanciesusingnon-invasiveprenataltestingDarineVillela1,2,HuiwenChe1,MarijkeVanGhelue1,3,4,LucDe