CK7/CK20 staining pattern and CDX2 expression were evaluated in ... files of the Department of Pathology, Fatih University Medical School, ...SkiptomaincontentAdvertisementSearchallBMCarticlesSearchDownloadPDFResearchOpenAccessPublished:23January2012ThevalueofCDX2andcytokeratins7and20expressionindifferentiatingcolorectaladenocarcinomasfromextraintestinalgastrointestinaladenocarcinomas:cytokeratin7-/20+phenotypeismorespecificthanCDX2antibodyReyhanBayrak1,HacerHaltas1&SibelYenidunya1 DiagnosticPathologyvolume 7,Article number: 9(2012)Citethisarticle59kAccesses92Citations2AltmetricMetricsdetailsAbstractBackground/ObjectiveMetastaticadenocarcinomafromanunknownprimarysiteisacommonclinicalproblem.Determiningthecytokeratin(CK)7/CK20patternoftumorsisoneofthemosthelpfulproceduresforthispurposesincetheCK7-/CK20+patternistypicalofcolorectaladenocarcinomas.CDX2,acriticalnucleartranscriptionfactorforintestinaldevelopment,isexpressedinintestinalepitheliumandadenocarcinomas.Inthepresentstudy,wecomparedthesensitivityandspecificityofCDX2expressionandtheCK7-/CK20+phenotypeindifferentiatingcolorectaladenocarcinomasfrompancreaticandgastricadenocarcinomas.MethodsCK7/CK20stainingpatternandCDX2expressionwereevaluatedin118casesofcolorectal,59casesofgastric,and32casesofpancreaticadenocarcinomas.Thesensitivity,specificity,andpositiveandnegativepredictivevaluesoftheCK7-/CK20+phenotypeandofCDX2expressionwereanalyzed.ResultsTheCK7-/CK20+immunophenotypewasexpressedby75of118(64%)colorectaland3of59(5%)gastrictumorsandwasnotobservedinanypancreaticadenocarcinomas.TheCK7+/CK20+immunophenotypewasexpressedin24/118(20%)ofcolon,28/59(48%)ofgastricand7/32(22%)ofpancreaticadenocarcinomas.TheCK7+/CK20-expressionpatternwasobservedinonly2%(2of118)ofcolorectalcarcinomas.CDX2wasexpressedin114of118(97%)colorectal,36of59(61%)gastric,and5of32(16%)pancreaticadenocarcinomas.TherewasnosignificantassociationbetweenCDX2expressionandtumordifferentiationincolorectalcarcinomas.Ingastriccarcinomas,CDX2expressionwasmorecomm