Several methods are effectual for diagnosis of scrub typhus that includes enzyme-linked immunosorbent assay (ELISA), immunofluorescence assay ( ...NCBISkiptomaincontentSkiptonavigationResourcesHowToAboutNCBIAccesskeysMyNCBISignintoNCBISignOutTryoutPMCLabsandtelluswhatyouthink.LearnMore.JournalList3Biotechv.10(9);2020SepPMC74315543Biotech.2020Sep;10(9):396.Publishedonline2020Aug18.doi: 10.1007/s13205-020-02389-wPMCID:PMC7431554PMID:32834918Diagnosisofscrubtyphus:recentadvancementsandchallengesDeepakKala,1ShagunGupta,2RupakNagraik,2VivekVerma,2AtulThakur,1andAnkurKaushal1DeepakKala1AmityCenterofNanotechnology,AmityUniversity,Haryana,122413IndiaFindarticlesbyDeepakKalaShagunGupta2ShooliniUniversity,Solan,173229IndiaFindarticlesbyShagunGuptaRupakNagraik2ShooliniUniversity,Solan,173229IndiaFindarticlesbyRupakNagraikVivekVerma2ShooliniUniversity,Solan,173229IndiaFindarticlesbyVivekVermaAtulThakur1AmityCenterofNanotechnology,AmityUniversity,Haryana,122413IndiaFindarticlesbyAtulThakurAnkurKaushal1AmityCenterofNanotechnology,AmityUniversity,Haryana,122413IndiaFindarticlesbyAnkurKaushalAuthorinformationArticlenotesCopyrightandLicenseinformationDisclaimer1AmityCenterofNanotechnology,AmityUniversity,Haryana,122413India2ShooliniUniversity,Solan,173229IndiaDeepakKala,Email:moc.liamg@uc09hcetoibkapeed.ContributorInformation.Correspondingauthor.Received2020May26;Accepted2020Aug10.Copyright©KingAbdulazizCityforScienceandTechnology2020ThisarticlehasbeencitedbyotherarticlesinPMC.Scrubtyphusisamite-borne,acutefebrileillnesscausedbythebacteriumOrientiatsutsugamushi.Itisare-emerginginfectiousdiseaseofthetsutsugamushitriangle.Scrubtyphusistransmittedthroughbitesofcontaminatedchiggers(larvalstage).Diagnosisofscrubtyphusischallengingasitssymptomsmimicwithotheracutefebrileillnesses.Severalmethodsareeffectualfordiagnosisofscrubtyphusthatincludesenzyme-linkedimmunosorbentassay(ELISA),immunofluorescenceassay(IFA),immunochromatographictest(ICT),Weil–Felix,polymerasechainreaction(PCR)andloop-medi