The mainstay in scrub-typhus diagnostics remains serology. The oldest test in current use is the Weil–Felix OX-K agglutination reaction, ...NCBISkiptomaincontentSkiptonavigationResourcesHowToAboutNCBIAccesskeysMyNCBISignintoNCBISignOutTryoutPMCLabsandtelluswhatyouthink.LearnMore.JournalListAmJTropMedHygv.82(3);2010MarPMC2829893AmJTropMedHyg.2010Mar;82(3):368–370.doi: 10.4269/ajtmh.2010.09-0233PMCID:PMC2829893PMID:20207857DiagnosisofScrubTyphusReviewedbyGavinC.K.W.Koh,*RichardJ.Maude,DanielH.Paris,PaulN.Newton,andStuartD.BlacksellAuthorinformationArticlenotesCopyrightandLicenseinformationDisclaimerFacultyofTropicalMedicine,MahidolUniversity,Bangkok,Thailand;DepartmentofMedicine,UniversityofCambridge,Cambridge,UnitedKingdom;CenterforExperimentalandMolecularMedicine,AcademicMedicalCenter,UniversityofAmsterdam,Amsterdam,TheNetherlands;CentreforClinicalVaccinologyandTropicalMedicine,NuffieldDepartmentofClinicalMedicine,UniversityofOxford,Oxford,UnitedKingdom;WellcomeTrust–MahosotHospital–OxfordTropicalMedicineResearchCollaboration,MahosotHospital,Vientiane,LaoPeople'sDemocraticRepublic*AddresscorrespondencetoGavinC.K.W.Koh,Mahidol–OxfordTropicalMedicineResearchUnit,FacultyofTropicalMedicine,MahidolUniversity,420/6RajvithiRoad,Bangkok10400,Thailand.E-mail:[email protected];Accepted2009Nov27.Copyright©TheAmericanSocietyofTropicalMedicineandHygieneThisisanOpenAccessarticledistributedunderthetermsoftheAmericanSocietyofTropicalMedicineandHygiene'sRe-useLicensewhichpermitsunrestrictednon-commercialuse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperlycited.ThisarticlehasbeencitedbyotherarticlesinPMC.ScrubtyphusistransmittedbytrombiculidmitesandisendemictoEastandSoutheastAsiaandNorthernAustralia.Theclinicalsyndromeclassicallyconsistsofafever,rash,andeschar,butscrubtyphusalsocommonlypresentsasanundifferentiatedfeverthatrequireslaboratoryconfirmationofthediagnosis,usuallybyindirectfluorescentantibody(IFA)assay.WediscussthelimitationsofIFA,