Symptoms of Staphylococcus lugdunensis infection are often similar to those of Staphylococcus aureus infection, including skin and ...SkiptomaincontentThankyouforvisitingnature.com.YouareusingabrowserversionwithlimitedsupportforCSS.Toobtainthebestexperience,werecommendyouuseamoreuptodatebrowser(orturnoffcompatibilitymodeinInternetExplorer).Inthemeantime,toensurecontinuedsupport,wearedisplayingthesitewithoutstylesandJavaScript.AdvertisementnaturescientificreportsarticlesarticleCompletegenomesequenceofamethicillin-resistantStaphylococcuslugdunensisstrainandcharacteristicsofitsstaphylococcalcassettechromosomemecDownloadPDFSubjectsMedicalresearchMicrobiologyMolecularbiologyAbstractSymptomsofStaphylococcuslugdunensisinfectionareoftensimilartothoseofStaphylococcusaureusinfection,includingskinandsoft-tissuelesions,bacteremiaandinfectiveendocarditis.Despitetheseverityoftheseinfections,S.lugdunensisisregardedasalessimportantpathogenthandrug-resistantS.aureus.Toinvestigateitsabilitytocauseinfectiousdiseases,amethicillin-resistantS.lugdunensis(MRSL)strainJICS135wasisolatedfromapatientwithbacteremiaandsubjectedtowholegenomesequencing.Similartomoststrainsofmethicillin-resistantS.aureus(MRSA),thisMRSLstrainpossessedthestaphylococcalcassettechromosomemec(SCCmec)locatedclosetotheoriginofreplication.However,theSCCmecinthisMRSLstrain,withthreeccrcomplexes,wasstructurallyuniqueandcurrentlyuntypable.Moreover,theSCCmecofthisMRSLstrainwasfoundtocarrytwogenesencodingmicrobialsurfacecomponentsrecognizingadhesivematrixmolecules(MSCRAMM)-likeproteinsaccompaniedbyglycosyltransferases,oneofwhichmayhavebeenderivedfromS.aureusandtheotherfromS.epidermidis,indicatingthatthisMRSLevolvedtocarryvirulencefactorsfromotherstaphylococci.Theemergenceofthisstrain,thefirstMRSLstrainwhosegenomehasbeensequencedcompletely,maybeofpublicconcern.DownloadPDFIntroductionIn1988,twonewcoagulase-negativespecies,StaphylococcuslugdunensisandS.schleiferi,wereisolatedfromhumanclinicalspecimens1.Oneofthesehumanpathogens,S.