PDF | Multidrug-resistant Klebsiella pneumoniae and Escherichia coli isolates harboring New Delhi metallo-β-lactamase (NDM-1) were isolated from a.ArticlePDFAvailableNewDelhiMetallo-β-LactamaseinKlebsiellapneumoniaeandEscherichiacoli,CanadaJanuary2011EmergingInfectiousDiseases17(1):103-6DOI:10.3201/eid1701.101358SourcePubMedAuthors:MichaelRichardMulveyGovernmentofCanadaJenniferGrantUniversityofBritishColumbia-VancouverKatherinePlewesMahidolOxfordTropicalMedicineResearchUnit(MORU)DianeLRoscoeVancouverGeneralHospitalShowall5authorsHideDownloadfull-textPDFDownloadfull-textPDFReadfull-textDownloadfull-textPDFDownloadfull-textPDFReadfull-textDownloadcitationCopylinkLinkcopiedReadfull-textDownloadcitationCopylinkLinkcopiedCitations(146)References(21)Figures(4)AbstractandFiguresMultidrug-resistantKlebsiellapneumoniaeandEscherichiacoliisolatesharboringNewDelhimetallo-β-lactamase(NDM-1)wereisolatedfromapatientwhohadreturnedtoCanadafromIndia.TheNDM-1genewasfoundoncloselyrelatedincompatibilitygroupA/Ctypeplasmids.TheoccurrenceofNDM-1inNorthAmericaisamajorpublichealthconcern.Macrorestrictionanalysis(XbaI)bypulsed-fieldgelelectrophoresisofKlebsiellapneumoniaeN100469(lane1),K.pneumoniaeN10-0506(lane2),EscherichiacoliN10-0505(lane3),E.coliN10-0705(lane4),SalmonellaentericaserovarBranderupmolecularmassmarker(XbaI)(lanesSB).… .PrimersusedinstudyofNewDelhimetallo--lactamaseinKlebsiellapneumoniaeandEscherichiacoli,Canada,2010… BglIIrestrictiondigestsofNewDelhimetallo-β-lactamase(pNDM)–Kp10469(lane1)andpNDM-Ec10505(lane2).MW,1-kbextensionladdermolecularmassmarker(Invitrogen,Carlsbad,CA,USA).Sizes(kb)areindicatedforsomebands.… .AntimicrobialdrugsusceptibilitiesoftheisolatesfromstudyofNewDelhimetallo--lactamaseinKlebsiellapneumoniaeandEscherichiacoli,Canada,2010*Test/antimicrobialagentK.pneumoniaeN10-0469E.coliDH10B(pNDM-Kp10469)E.coliN10-0505E.coliDH10B(pNDM-Ec10505)E.coliDH10B… Figures-uploadedbyDavidABoydAuthorcontentAllfigurecontentinthisareawasuploadedbyDavidABoydContentmaybesubject