NCBISkiptomaincontentSkiptonavigationResourcesHowToAboutNCBIAccesskeysMyNCBISignintoNCBISignOutJournalListApplEnvironMicrobiolv.69(1);2003JanPMC152381ApplEnvironMicrobiol.2003Jan;69(1):350–357.doi: 10.1128/AEM.69.1.350-357.2003PMCID:PMC152381PMID:12514015CapsidFunctionsofInactivatedHumanPicornavirusesandFelineCalicivirusSuphachaiNuanualsuwanandDeanO.Cliver*SuphachaiNuanualsuwanDepartmentofPopulationHealthandReproduction,SchoolofVeterinaryMedicine,UniversityofCalifornia,Davis,California95616-8743FindarticlesbySuphachaiNuanualsuwanDeanO.CliverDepartmentofPopulationHealthandReproduction,SchoolofVeterinaryMedicine,UniversityofCalifornia,Davis,California95616-8743FindarticlesbyDeanO.CliverAuthorinformationArticlenotesCopyrightandLicenseinformationDisclaimerDepartmentofPopulationHealthandReproduction,SchoolofVeterinaryMedicine,UniversityofCalifornia,Davis,California95616-8743*Correspondingauthor.Mailingaddress:DepartmentofPopulationHealthandReproduction,SchoolofVeterinaryMedicine,UniversityofCalifornia,Davis,California95616-8743.Phone:(530)754-9120.Fax:(530)752-5845.E-mail:[email protected];Accepted2002Oct10.Copyright©2003,AmericanSocietyforMicrobiologyThisarticlehasbeencitedbyotherarticlesinPMC.Theexceptionalstabilityofentericvirusesprobablyresidesintheircapsids.Thecapsidfunctionsofinactivatedhumanpicornavirusesandfelinecalicivirus(FCV)weredetermined.ViruseswereinactivatedbyUV,hypochlorite,hightemperature(72°C),andphysiologicaltemperature(37°C),allofwhicharepertinenttotransmissionviafoodandwater.Poliovirus(PV)andhepatitisAvirus(HAV)aretransmissibleviawaterandfood,andFCVisthebestavailablesurrogatefortheNorwalk-likeviruses,whichareleadingcausesoffood-borneandwaterbornediseaseintheUnitedStates.Thecapsidsofall37°C-inactivatedvirusesstillprotectedtheviralRNAagainstRNase,eveninthepresenceofproteinaseK,whichcontrastedwithfindingswithvirusesinactivatedat72°C.Thelossofabilityofthevirustoattachtohomologouscellreceptorswasuniversal,regardlessofvirustypeandinact