spiralis, T. britovi and T. pseudospiralis infections, were also included. Results. Sera from mice infected with one Trichinella species reacted ...SkiptomaincontentAdvertisementSearchallBMCarticlesSearchDownloadPDFResearchOpenAccessPublished:12December2018DifferentiationofTrichinellaspecies(Trichinellaspiralis/TrichinellabritoviversusTrichinellapseudospiralis)usingwesternblotMariaAngelesGómez-Morales1,AlessandraLudovisi1,MarcoAmati1,SimonaCherchi1,DanieleTonanzi1&EdoardoPozio1 Parasites&Vectorsvolume 11,Article number: 631(2018)Citethisarticle3102Accesses8Citations1AltmetricMetricsdetailsAbstractBackgroundTrichinellosisisameat-bornezoonoticdiseasecausedbyparasitesofthegenusTrichinella.Todate,12taxahavebeendescribed.TheidentificationofTrichinellaspeciesiscrucialinordertoidentifythepossiblesourceofinfection,thegeographicaloriginoftheparasiteandtoassessriskofinfectionfordomesticpigsandhumans.Specificidentificationoftheetiologicalagentisnotalwaysfeasibleusingdirectmethodssincethesourceofinfectioncanbeuntraceable.TheaimofthisstudywastodevelopadiagnostictooltoinferthecausativeTrichinellaspeciesusingwesternblotpatternsofseraderivedfrominfectedanimalandhumanhosts.MethodsSerafrommiceexperimentallyinfectedwithTrichinellaspiralis,Trichinellabritovi,TrichinellapseudospiralisandTrichinellapapuaeweretestedbywesternblotusinghomologousandheterologouscrudewormextracts(CWE)andahighlysensitivedetectionsystembasedonchemiluminescence.Inaddition,serafrompigsexperimentallyinfectedwithT.spiralis,T.britoviandT.pseudospiralisandfrompatientswithconfirmedT.spiralis,T.britoviandT.pseudospiralisinfections,werealsoincluded.ResultsSerafrommiceinfectedwithoneTrichinellaspeciesreactedwithCWEproteinsfromallfourinvestigatedspecies.Likewise,seraderivedfrompigsandhumansinfectedwithoneTrichinellaspeciesreactedwithCWEproteinsfromallthethreeinvestigatedspecies.UsingT.spiralisCWE,serafromT.pseudospiralis-infectedhostsyieldedacharacteristicpatternofreactivityusingWb,whichdifferedtothatproducedbyT.spiralis/T