The aim of this study was to investigate the biological properties of a Trichinella spiralis PPase (TsPPase) and its role in larval molting and ...SkiptomaincontentAdvertisementSearchallBMCarticlesSearchDownloadPDFResearcharticleOpenAccessPublished:07January2021BiologicalpropertiesandrolesofaTrichinellaspiralisinorganicpyrophosphataseinmoltinganddevelopmentalprocessofintestinallarvalstagesChenXiHu1,JieZeng1,HuiNanHao1,YangXiuYueXu1,FangLiu1,RuoDanLiu1,ShaoRongLong1,ZhongQuanWang1&JingCui ORCID:orcid.org/0000-0001-8726-54981 VeterinaryResearchvolume 52,Article number: 6(2021)Citethisarticle880Accesses2AltmetricMetricsdetailsAbstractInorganicpyrophosphatase(PPase)participatesinenergycycleandplaysavitalroleinhydrolysisofinorganicpyrophosphate(PPi)intoinorganicphosphate(Pi).TheaimofthisstudywastoinvestigatethebiologicalpropertiesofaTrichinellaspiralisPPase(TsPPase)anditsroleinlarvalmoltinganddevelopmentalprocess.ThepredictedTsPPaseconsistedof367aminoacidswithamolecularmassof41.48 kDaandapIof5.76.AminoacidsequencealignmentandphylogeneticanalysisshowedthattheTsPPasegeneencodesafunctionalfamilyIsolublePPasewiththesamecharacteristicsasprokaryotic,plantandanimal/fungalsolublePPase.TherTsPPasewasexpressedandpurified,ithastheactivitytocatalyzethehydrolysisofPPitoPi,andtheactivitywasdependentonMg2+,pHandtemperature.TheenzymaticactivityofrTsPPasewassignificantlyinhibitedafteritsmetalbindingsitesmutation.TsPPasewastranscribedandexpressedinallT.spiralisphases,especiallyinmusclelarvae(ML)andintestinalinfectivelarvae(IIL).Immunofluorescenceassay(IFA)revealedthatTsPPasewasmainlylocatedincuticleandstichosome.WhentheMLandIILweretreatedwithTsPPase-specificsiRNA-279,TsPPaseexpressionandenzymaticactivitywereobviouslyreduced,thelarvalmoltinganddevelopmentwerealsoimpeded.IntestinalIILaswellasAWburden,IILmoltingratesfrommiceinfectedwithsiRNA-treatedMLwereobviouslysuppressed.TheresultsindicatedthatrTsPPasepossessestheenzymaticactivityofnativeinorganicpyrophosphatase,andTsPPaseplaysanimportant