Diagnostic accuracy of an in-house Scrub Typhus enzyme ... | scrub typhus igm positive means

ResultsArticleAuthorsMetricsCommentsMediaCoverageReaderComments(0)FiguresFiguresAbstractScrubtyphusisamajorcauseofmorbidityandmortalityinSoutheastAsia.Diagnosisofscrubtyphusisdifficultduetoalackofaccessiblevalidateddiagnostictools.Despiteitsobjectivity,thediagnosticaccuracyofELISAtestsisinfluencedbymethodologicalandpatientfactors.Thisstudyaimstoevaluatetheperformanceofanovelin-houseELISAdevelopedintheMahidolOxfordTropicalMedicineResearchUnit(MORU)foranti-scrubtyphusgroupIgMandIgGcomparedtothe“goldstandard”referenceIFAandPCR,andtodeterminewhetherthein-houseELISAcanbeusedasaseroepidemiologicalscreeningtooland/orstand-alonetestforscrubtyphus.Atotalof1,976admissionand1,438participantfollow-upseracollectedintheLaoPDR(Laos)weretestedwithELISAforIgMandIgG.SampleswithanELISAOD≥0.50weretestedwithIFAforIgMand/orIgG.AstrongpositiverelationshipwaspresentbetweenELISAODsandIFAtitersforadmissionIgM(r2:0.70,p<0.005)andIgG(r2:0.76,p<0.005),andforfollow-upIgMandIgG(bothr2:0.76,p<0.005)samples.ThebestcompromisebetweensensitivityandspecificityfortheELISAODcut-offislikelytobebetween0.8–1.0forIgMantibodiesand1.2–1.8forIgGantibodies.TheseresultsdemonstratethatthediagnosticaccuracyoftheMORUin-housescrubtyphusgroupELISAiscomparabletothatofIFA,withsimilarresultsasreportedforthecommonlyusedInBiosScrubTyphusDetectELISA,validatingtheuseofthein-houseELISA.TheoptimalELISAcut-offwoulddependontheuseofthetest,andthedesiredsensitivityandspecificity.Furtherstudiesarerequiredtoauthenticatetheuseofthesecut-offsinotherendemicregions.Thisin-houseELISAhasthepotentialtoreplacetheimperfectIFA,whichcouldultimatelyreducetheburdenofscrubtyphusbyimprovingtherateofscrubtyphusdiagnosesinendemiclow-resourceareas.AuthorsummaryScrubtyphusisamite-borneinfectiousdisease,causedbytheOrientiatsutsugamushibacterium,resultinginconsiderablemorbidityandmortalityintheAsia-Pacificregion.Diagnosisisdifficultsinceitdoesnotpresentwithanydistinctiveclinicalsignscomparedtootherfebrileillnessesinthisregion,exceptforanecroticskinlesio

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Scrub Typhus IgM ELISA | scrub typhus igm positive means
* Laboratory results can never be the only base of a medical report. The patient history and further tests have to be taken into account. Test.
SCRUB TYPHUS (TSUTSUGAMUSHI) IgM & IgG CARD | scrub typhus igm positive means
detection of IgM and IgG antibodies to Scrub Typhus antibodies in human serum ... considered as positive for that particular antibody type (IgM or IgG).
Kinetics of IgM and IgG antibodies after scrub typhus infection ... | scrub typhus igm positive means
Methods: Adult patients previously confirmed to have scrub typhus by IgM ELISA, positive PCR, or both, were included in this cross-sectional study.
Antibody response following scrub typhus infection | scrub typhus igm positive means
Results
Diagnostic accuracy of an in-house Scrub Typhus enzyme ... | scrub typhus igm positive means
Results
Scrub Typhus | scrub typhus igm positive means
Treatment · Scrub typhus should be treated with the antibiotic doxycycline. Doxycycline can be used in persons of any age. · Antibiotics are most effective if ...
Kinetics of IgM and IgG antibodies after scrub typhus infection ... | scrub typhus igm positive means
Clinicians need to be cautious in using a single serum sample for the detection of IgM to diagnose scrub typhus, as it remains elevated for up to 12 months ...
Diagnostic evaluation of IgM ELISA and IgM ... | scrub typhus igm positive means
Scrub typhus is an acute febrile illness caused by the obligate intracellular bacteria Orientia tsutsugamushi. Scrub typhus is an arthropod- ...
Kinetics of IgM and IgG antibodies after scrub typhus ... | scrub typhus igm positive means
Results
Human Scrub Typhus IgM ELISA kit (DEIABL75) | scrub typhus igm positive means