Lassa virus is a segmented negative-strand RNA virus of the family Arenaviridae. It belongs to the Old World complex of the arenaviruses, which also includes the ...SkiptomaincontentStructureandAssemblyDomainStructureofLassaVirusLProteinLindaBrunotte,MichaelaLelke,MeikeHass,KatjaKleinsteuber,BeateBecker-Ziaja,StephanGüntherLindaBrunotteDepartmentofVirology,Bernhard-Nocht-InstituteforTropicalMedicine,20359Hamburg,GermanyFindthisauthoronGoogleScholarFindthisauthoronPubMedSearchforthisauthoronthissiteMichaelaLelkeDepartmentofVirology,Bernhard-Nocht-InstituteforTropicalMedicine,20359Hamburg,GermanyFindthisauthoronGoogleScholarFindthisauthoronPubMedSearchforthisauthoronthissiteMeikeHassDepartmentofVirology,Bernhard-Nocht-InstituteforTropicalMedicine,20359Hamburg,GermanyFindthisauthoronGoogleScholarFindthisauthoronPubMedSearchforthisauthoronthissiteKatjaKleinsteuberDepartmentofVirology,Bernhard-Nocht-InstituteforTropicalMedicine,20359Hamburg,GermanyFindthisauthoronGoogleScholarFindthisauthoronPubMedSearchforthisauthoronthissiteBeateBecker-ZiajaDepartmentofVirology,Bernhard-Nocht-InstituteforTropicalMedicine,20359Hamburg,GermanyFindthisauthoronGoogleScholarFindthisauthoronPubMedSearchforthisauthoronthissiteStephanGüntherDepartmentofVirology,Bernhard-Nocht-InstituteforTropicalMedicine,20359Hamburg,GermanyFindthisauthoronGoogleScholarFindthisauthoronPubMedSearchforthisauthoronthissiteForcorrespondence:[email protected]: 10.1128/JVI.00721-10ArticleFigures&DataInfo&MetricsPDFABSTRACTThe200-kDaLproteinofarenavirusesplaysacentralroleinviralgenomereplicationandtranscription.ThisstudyaimedatprovidingevidenceforthedomainstructureofLproteinbycombiningbioinformaticswithastepwisemutagenesisapproachusingtheLassavirusminirepliconsystem.Potentialinterdomainlinkerswerepredictedusingvariousalgorithms.Thepredictionwaschallengedbyinsertionofflexiblesequencesintothepredictedlinkers.Insertionof5or10aminoacidresidueswastoleratedatsevensites(S407,G446,G467,G774,G939,S1952,andV2074in