AmpC β-lactamase-producing Enterobacterales: what a clinician should know. Infection. 2019 Jun;47(3):363-375. doi: ...Clipboard,SearchHistory,andseveralotheradvancedfeaturesaretemporarilyunavailable.SkiptomainpagecontentCOVID-19InformationPublichealthinformation(CDC)Researchinformation(NIH)SARS-CoV-2data(NCBI)Preventionandtreatmentinformation(HHS)Español AccesskeysNCBIHomepageMyNCBIHomepageMainContentMainNavigationSearch:SearchAdvancedClipboardUserGuideSaveEmailSendtoClipboardMyBibliographyCollectionsCitationmanagerDisplayoptionsDisplayoptionsFormatAbstractPubMedPMIDSavecitationtofileFormat:Summary(text)PubMedPMIDAbstract(text)CSVCreatefileCancelEmailcitationSubject:1selecteditem:30840201-PubMedTo:Format:SummarySummary(text)AbstractAbstract(text)MeSHandotherdataSendemailCancelAddtoCollectionsCreateanewcollectionAddtoanexistingcollectionNameyourcollection:Namemustbelessthan100charactersChooseacollection:UnabletoloadyourcollectionduetoanerrorPleasetryagainAddCancelAddtoMyBibliographyMyBibliographyUnabletoloadyourdelegatesduetoanerrorPleasetryagainAddCancelYoursavedsearchNameofsavedsearch:Searchterms:TestsearchtermsWouldyoulikeemailupdatesofnewsearchresults?SavedSearchAlertRadioButtonsYesNoEmail:(change)Frequency:MonthlyWeeklyDailyWhichday?ThefirstSundayThefirstMondayThefirstTuesdayThefirstWednesdayThefirstThursdayThefirstFridayThefirstSaturdayThefirstdayThefirstweekdayWhichday?SundayMondayTuesdayWednesdayThursdayFridaySaturdayReportformat:SummarySummary(text)AbstractAbstract(text)PubMedSendatmost:1item5items10items20items50items100items200itemsSendevenwhentherearen'tanynewresultsOptionaltextinemail:SaveCancelCreateafileforexternalcitationmanagementsoftwareCreatefileCancelYourRSSFeedNameofRSSFeed:Numberofitemsdisplayed:510152050100CreateRSSCancelRSSLinkCopyFulltextlinksSpringerFulltextlinksActionsCiteFavoritesDisplayoptionsDisplayoptionsFormatAbstractPubMedPMIDSharePermalinkCopyPagenavigationTitle&authorsAbstractSimilararticlesCitedbyReferencesPublicationtypesMeSHterms

Clipboard,SearchHistory,andseveralotheradvancedfeaturesaretemporarilyunavailable.SkiptomainpagecontentCOVID-19InformationPublichealthinformation(CDC)Researchinformation(NIH)SARS-CoV-2data(NCBI)Preventionandtreatmentinformation(HHS)Español AccesskeysNCBIHomepageMyNCBIHomepageMainContentMainNavigationSearch:SearchAdvancedClipboardUserGuideSaveEmailSendtoClipboardMyBibliographyCollectionsCitationmanagerDisplayoptionsDisplayoptionsFormatAbstractPubMedPMIDSavecitationtofileFormat:Summary(text)PubMedPMIDAbstract(text)CSVCreatefileCancelEmailcitationSubject:1selecteditem:19136439-PubMedTo:Format:SummarySummary(text)AbstractAbstract(text)MeSHandotherdataSendemailCancelAddtoCollectionsCreateanewcollectionAddtoanexistingcollectionNameyourcollection:Namemustbelessthan100charactersChooseacollection:UnabletoloadyourcollectionduetoanerrorPleasetryagainAddCancelAddtoMyBibliographyMyBibliographyUnabletoloadyourdelegatesduetoanerrorPleasetryagainAddCancelYoursavedsearchNameofsavedsearch:Searchterms:TestsearchtermsWouldyoulikeemailupdatesofnewsearchresults?SavedSearchAlertRadioButtonsYesNoEmail:(change)Frequency:MonthlyWeeklyDailyWhichday?ThefirstSundayThefirstMondayThefirstTuesdayThefirstWednesdayThefirstThursdayThefirstFridayThefirstSaturdayThefirstdayThefirstweekdayWhichday?SundayMondayTuesdayWednesdayThursdayFridaySaturdayReportformat:SummarySummary(text)AbstractAbstract(text)PubMedSendatmost:1item5items10items20items50items100items200itemsSendevenwhentherearen'tanynewresultsOptionaltextinemail:SaveCancelCreateafileforexternalcitationmanagementsoftwareCreatefileCancelYourRSSFeedNameofRSSFeed:Numberofitemsdisplayed:510152050100CreateRSSCancelRSSLinkCopyFulltextlinksAtyponFreePMCarticleFulltextlinksActionsCiteFavoritesDisplayoptionsDisplayoptionsFormatAbstractPubMedPMIDSharePermalinkCopyPagenavigationTitle&authorsAbstractFiguresSimilararticlesCitedbyPublicationtypesMeSHtermsSubstancesRelatedinformationLinkOut-moreresourcesTitle&authorsAbstractFiguresSimilararticlesCitedbyPublicatio

Exposure to β-lactams can trigger a cascade of events leading to significant AmpC production and β-lactam resistance, even for infections caused by initially ...SkiptoMainContentAdvertisementSearchMenuAccountMenuMenuSignInRegisterNavbarSearchFilterThisissueAllClinicalInfectiousDiseasesAllIDSAJournalsAllJournalsMobileMicrositeSearchTermSearchSignInRegisterIssuesMoreContentAdvancearticlesEditor'sChoiceSupplementArchiveCoverArchiveIDSAGuidelinesIDSAJournalsTheJournalofInfectiousDiseasesOpenForumInfectiousDiseasesPhotoQuizzesPublishAuthorGuidelinesSubmitOpenAccessPurchaseAdvertiseAdvertisingandCorporateServicesAdvertisingJournalsCareerNetworkMediakitReprintsandePrintsSponsoredSupplementsBrandedBooksAboutAboutClinicalInfectiousDiseasesAbouttheInfectiousDiseasesSocietyofAmericaAbouttheHIVMedicineAssociationIDSACOIPolicyEditorialBoardAlertsSelf-ArchivingPolicyForReviewersForPressOfficesIssuesMoreContentAdvancearticlesEditor'sChoiceSupplementArchiveCoverArchiveIDSAGuidelinesIDSAJournalsTheJournalofInfectiousDiseasesOpenForumInfectiousDiseasesPhotoQuizzesPublishAuthorGuidelinesSubmitOpenAccessPurchaseAdvertiseAdvertisingandCorporateServicesAdvertisingJournalsCareerNetworkMediakitReprintsandePrintsSponsoredSupplementsBrandedBooksAboutAboutClinicalInfectiousDiseasesAbouttheInfectiousDiseasesSocietyofAmericaAbouttheHIVMedicineAssociationIDSACOIPolicyEditorialBoardAlertsSelf-ArchivingPolicyForReviewersForPressOfficesClosesearchfilterThisissueAllClinicalInfectiousDiseasesAllIDSAJournalsAllJournalssearchinputSearchAdvancedSearchSearchMenuSkipNavDestinationArticleNavigationClosemobilesearchnavigationArticleNavigationVolume69Issue815October2019ArticleContentsAbstractMECHANISMSOFRESISTANCETRIGGERSOFAmpCHYPEREXPRESSIONSPECIES-SPECIFICEPIDEMIOLOGYOFAmpCPRODUCTIONMETHODSOFAmpCβ-LACTAMASEDETECTIONTREATMENTOPTIONSCONCLUSIONSNotesReferencesArticleNavigationEditor'sChoiceAPrimeronAmpCβ-Lactamases:NecessaryKnowledgeforanIncreasinglyMultidrug-resistantWorldPranitaDTamma,PranitaD

NCBISkiptomaincontentSkiptonavigationResourcesHowToAboutNCBIAccesskeysMyNCBISignintoNCBISignOutTryoutPMCLabsandtelluswhatyouthink.LearnMore.JournalListJLabPhysiciansv.6(1);Jan-Jun2014PMC3969634JLabPhysicians.2014Jan-Jun;6(1):1–6.doi: 10.4103/0974-2727.129082PMCID:PMC3969634PMID:24696552DetectionofAmpCβLactamasesinGram-negativeBacteriaGunjanGupta,VibhorTak,andPurvaMathurGunjanGuptaDepartmentofLaboratoryMedicine,JaiPrakashNarainApexTraumaCentre,AllIndiaInstituteofMedicalSciences,NewDelhi,IndiaFindarticlesbyGunjanGuptaVibhorTakDepartmentofLaboratoryMedicine,JaiPrakashNarainApexTraumaCentre,AllIndiaInstituteofMedicalSciences,NewDelhi,IndiaFindarticlesbyVibhorTakPurvaMathurDepartmentofLaboratoryMedicine,JaiPrakashNarainApexTraumaCentre,AllIndiaInstituteofMedicalSciences,NewDelhi,IndiaFindarticlesbyPurvaMathurAuthorinformationCopyrightandLicenseinformationDisclaimerDepartmentofLaboratoryMedicine,JaiPrakashNarainApexTraumaCentre,AllIndiaInstituteofMedicalSciences,NewDelhi,IndiaAddressforcorrespondence:Dr.PurvaMathur,E-mail:ni.oc.oohay@ruhtamavrupCopyright:©JournalofLaboratoryPhysiciansThisisanopen-accessarticledistributedunderthetermsoftheCreativeCommonsAttribution-Noncommercial-ShareAlike3.0Unported,whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperlycited.ThisarticlehasbeencitedbyotherarticlesinPMC.AmpCβ-lactamasesareclinicallyimportantcephalosporinasesencodedonthechromosomesofmanyEnterobacteriaceaeandafewotherorganisms,wheretheymediateresistancetocephalothin,cefazolin,cefoxitin,mostpenicillins,andβ-lactamaseinhibitor/β-lactamcombinations.TheincreaseinantibioticresistanceamongGram-negativebacteriaisanotableexampleofhowbacteriacanprocure,maintainandexpressnewgeneticinformationthatcanconferresistancetooneorseveralantibiotics.Detectionoforganismsproducingtheseenzymescanbedifficult,becausetheirpresencedoesnotalwaysproducearesistantphenotypeonconventionaldiscdiffusionorautomatedsusceptibilitytestingmethods.Theseenzymesareoftena

The genes encoding AmpC β-lactamase have spread extensively and are widely detected in bacterial plasmids. The first plasmid-encoded AmpC ...JournalsWhyPublishWithUs?EditorialPoliciesAuthorGuidelinesPeerReviewGuidelinesOpenOutlookCOVID-19BlogReprintsSubmitNewManuscriptMenuAboutContactSustainabilityAwardsPressCenterTestimonialsBlogFavoredAuthorProgramPermissionsPre-SubmissionReprintsLoginopenaccesstoscientificandmedicalresearchAdvancedsearchHomeJournalsWhypublishwithus?EditorialPoliciesAuthorInformationPeerReviewGuidelinesOpenOutlookCOVID-19AverageArticleStatistics20Days*Fromsubmissiontofirsteditorialdecision.11Days*Fromeditorialacceptancetopublication.*Businessdays(Mon-Fri)RejectionRate61%Theabovepercentageofmanuscriptshavebeenrejectedinthelast12months.66581PapersPublishedSubmitNewManuscriptLogintoviewexistingmanuscriptstatusSignupforJournalalertsAboutDovePressOpenaccesspeer-reviewedscientificandmedicaljournals.LearnmoreOpenAccessDoveMedicalPressisamemberoftheOAI.LearnmoreReprintsBulkreprintsforthepharmaceuticalindustry.LearnmoreFavoredAuthorsWeofferrealbenefitstoourauthors,includingfast-trackprocessingofpapers.LearnmorePromotionalArticleMonitoringRegisteryourspecificdetailsandspecificdrugsofinterestandwewillmatchtheinformationyouprovidetoarticlesfromourextensivedatabaseandemailPDFcopiestoyoupromptly.LearnmoreSocialMedia    BacktoJournals»InfectionandDrugResistance»Volume13OriginalResearchPlasmid-MediatedAmpCβ-LactamaseCITMandDHAMGenesAmongGram-NegativeClinicalIsolates                  FulltextMetricsGetPermissionCitethisarticleAuthorsAryalSC,UpretiMK,SahAK,AnsariM,NepalK,DhungelB,AdhikariN,LekhakB,RijalKRReceived30September2020Acceptedforpublication6November2020Published24November2020Volume2020:13Pages4249—4261DOIhttps://doi.org/10.2147/IDR.S284751CheckedforplagiarismYesReviewbySingleanonymouspeerreviewPeerreviewercomments2Editorwhoapprovedpublication:DrHéctorM.Mora-MontesDownloadArticle[PDF] SubhasChandraAryal,1,*MilanKumarUpreti,1,*AnilKumarSah,2MeharajAnsari,3Kr

AmpC β-lactamases are clinically important cephalosporinases encoded on the chromosomes of many of the Enterobacteriaceae and a few other ...RequestUsernameCan'tsignin?Forgotyourusername?EnteryouremailaddressbelowandwewillsendyouyourusernameEmail*CloseIftheaddressmatchesanexistingaccountyouwillreceiveanemailwithinstructionstoretrieveyourusernameCreateanewaccountEmailReturninguserAmericanSocietyforMicrobiology("ASM")iscommittedtomaintainingyourconfidenceandtrustwithrespecttotheinformationwecollectfromyouonwebsitesownedandoperatedbyASM("ASMWebSites")andothersources.ThisPrivacyPolicysetsforththeinformationwecollectaboutyou,howweusethisinformationandthechoicesyouhaveabouthowweusesuchinformation.ACCEPT thecookiepolicyand& CONTINUEFINDOUTMOREabouttheprivacypolicyChangePasswordOldPasswordNewPasswordTooShortWeakMediumStrongVeryStrongTooLongPasswordChangedSuccessfullyYourpasswordhasbeenchangedLoginRegisterEmail*Password*Forgotpassword?ResetithereKeepmeloggedinFieldswith*aremandatoryDon'thaveanaccount?CreateonehereEmail*Fieldswith*aremandatoryAlreadyhaveanaccount?LoginhereCan'tsignin?Forgotyourpassword?EnteryouremailaddressbelowandwewillsendyoutheresetinstructionsEmail*Retypethecodefromthepicture: CancelIftheaddressmatchesanexistingaccountyouwillreceiveanemailwithinstructionstoresetyourpassword.CloseVerifyPhoneEntertheverificationcodeCancelCongrats!YourPhonehasbeenverifiedcloseCLOSEEnterwords/phrases/DOI/ISBN/keywords/authors/etcSearchSearchAdvancedsearchSuggestedTerms:authorinformationmigrationinformationpublicationfeesCOVID-19collectionasmjournalscollections

DirektzumInhaltspringenDirektzurServicenavigationspringen NavigationundServiceServicemenüHomepageSitemapContact-AA+AGermanSchnellsucheSearch advancedsearchA-ZIndexABCDEFGHIJKLMNOPQRSTUVWXYZSearchA-ZIndexHauptmenüTheInstituteMissionstatementFacts&FiguresRemitTheBfRstructureWorkingattheBfRScientificAdvisoryBoardPrinciplesofScientificPracticeDatabasesGLPFederalBureauQualitymanagementEnvironmentalManagementTheBfR-CommitteesCo-operationNationalBreastfeedingCommitteeReferenceLaboratoriesEFSAFocalPointGermanCentrefortheProtectionofLaboratoryAnimals(Bf3R)FoodsafetyMicrobialrisksSubstancerisksFeedsNutritionalrisksSpecialfoodgroupsProductSafetyFoodcontactmaterialsCosmeticsToysTextilesTobaccoproductsOtherconsumerproductsNanotechnologyChemicalSafetyREACH-thenewEuropeanchemicalslegislationBiocidalproductsandtreatedgoodsPlantprotectionproductsTransportofdangerousgoodsPoisoningsYouarehere:HomepageFAQESBLandAmpC-producingantimicrobialresistantbacteriaweitereThemendesBFRRiskcommunicationResearchScienceNewsPressofficePublicationsEventsoftheBfRAcademyFAQNewNewsletterRSS-FeedJobadvertisementNotepadQuestionsandanswersonESBLandAmpC-producingantimicrobialresistantbacteriaUpdatedFAQattheBfRof19January2015Bacteriacanberesistanttocertainantibiotics.Whenthishappenswithpathogenicbacteria,theseantibioticsremainineffectiveiftheyareusedtotreatanillnesscausedbythebacteria.Importantantimicrobialstowardswhichincreasingresistancehasbeenobservedincludethegroupofaminopenicillinsandcephalosporins.Oneofthecausesofthisantimicrobialresistanceareenzymesdesignatedas"extended-spectrumbeta-lactamases"(ESBL)and"ampCbeta-lactamases"(AmpC).Bacteriarequireacertain"resistancegene"inordertoproducetheseenzymes.Thisgeneticpropertycanbepassedonfromonebacteriagenerationtothenextthroughcelldivisionduringpropagation.Duringthelifetimeofabacterium,however,itcanalsobepassedonfromonebacteriacelltoanotherontransmissiblegenesectionssuchasplasmids.Thesetwocellscanalsobelongtodifferentbacteriatypes.Theuseofantibioticsinhumansanda

Phenotypic characterization of ESBLs and AmpC β-lactamases was performed utilizing ... Out of 311 GNB isolates, the frequency of ESBL and AmpC β-lactamase ...CanadianJournalofInfectiousDiseasesandMedicalMicrobiology+JournalMenuPDFJournaloverviewForauthorsForreviewersForeditorsTableofContentsSpecialIssuesSubmitCanadianJournalofInfectiousDiseasesandMedicalMicrobiology/2019/ArticleArticleSectionsOnthispageAbstractIntroductionMaterialsandMethodsResultsDiscussionConclusionDataAvailabilityConflictsofInterestAuthors’ContributionsAcknowledgmentsReferencesCopyrightResearchArticle|OpenAccessVolume2019|ArticleID6054694|https://doi.org/10.1155/2019/6054694MutasimE.Ibrahim,MohammedAbbas,AbdullahM.Al-Shahrai,BahaeldinK.Elamin,"PhenotypicCharacterizationandAntibioticResistancePatternsofExtended-Spectrumβ-Lactamase-andAmpCβ-Lactamase-ProducingGram-NegativeBacteriainaReferralHospital,SaudiArabia",CanadianJournalofInfectiousDiseasesandMedicalMicrobiology,vol.2019,ArticleID6054694,9pages,2019.https://doi.org/10.1155/2019/6054694ShowcitationPhenotypicCharacterizationandAntibioticResistancePatternsofExtended-Spectrumβ-Lactamase-andAmpCβ-Lactamase-ProducingGram-NegativeBacteriainaReferralHospital,SaudiArabiaMutasimE.Ibrahim,1MohammedAbbas,2AbdullahM.Al-Shahrai,3andBahaeldinK.Elamin1,41DepartmentofBasicMedicalScience(MicrobiologyUnit),CollegeofMedicine,UniversityofBisha,Bisha,SaudiArabia2DepartmentofPediatrics,CollegeofMedicine,UniversityofBisha,Bisha,SaudiArabia3DepartmentofFamilyMedicine,CollegeofMedicine,UniversityofBisha,Bisha,SaudiArabia4DepartmentofMedicalMicrobiology,FacultyofMedicalLaboratorySciences,UniversityofKhartoum,Khartoum,SudanShowmoreAcademicEditor:KapilChousalkarReceived05Feb2019Revised25May2019Accepted16Jun2019Published26Jun2019Background.Emergenceofpathogenicbacteriacarryingβ-lactamase-resistantdeterminantshasbecomeamajorhealthprobleminthehospitalsetting.Thestudyaimedtodetermineantibiotic-resistantpatternsandfrequencyofextended-spectrumβ-lactamase-(ESBL-)producingGram-

AmpC-type β-lactamases (class C)Beta-lactamaseFromWikipedia,thefreeencyclopediaJumptonavigationJumptosearchClassofenzymesBeta-lactamaseStructureofStreptomycesalbusbeta-lactamaseIdentifiersSymbolβ-lactamasedomainPfamPF00144PfamclanCL0013InterProIPR001466PROSITEPS00146SCOP256601/SCOPe/SUPFAMAvailableproteinstructures:Pfam structures/ECOD PDBRCSBPDB;PDBe;PDBjPDBsumstructuresummaryPDB1axb​,1blp​,1bsg​,1bue​,1e25​,1ghi​,1i2s​,1n9b​,1ong​,2cc1​,2gdn​,3dwz​β-lactamaseActionofβ-lactamaseanddecarboxylationoftheintermediateIdentifiersECno.3.5.2.6CASno.9073-60-3DatabasesIntEnzIntEnzviewBRENDABRENDAentryExPASyNiceZymeviewKEGGKEGGentryMetaCycmetabolicpathwayPRIAMprofilePDBstructuresRCSBPDBPDBePDBsumGeneOntologyAmiGO/QuickGOSearchPMCarticlesPubMedarticlesNCBIproteinsCorestructureofpenicillins(top)andcephalosporins(bottom).β-lactamringinred.Escherichiacolibacteriaontherightaresensitivetotwobeta-lactamantibiotics,anddonotgrowinthesemi-circularregionssurroundingantibiotics.E.colibacteriaontheleftareresistanttobeta-lactamantibiotics,andgrownexttooneantibiotic(bottom)andarelessinhibitedbyanotherantibiotic(top).Beta-lactamasesareenzymes(EC3.5.2.6)producedbybacteriathatprovidemulti-resistancetoβ-lactamantibioticssuchaspenicillins,cephalosporins,cephamycins,andcarbapenems(ertapenem),althoughcarbapenemsarerelativelyresistanttobeta-lactamase.Beta-lactamaseprovidesantibioticresistancebybreakingtheantibiotics'structure.Theseantibioticsallhaveacommonelementintheirmolecularstructure:afour-atomringknownasaβ-lactam.Throughhydrolysis,theenzymelactamasebreakstheβ-lactamringopen,deactivatingthemolecule'santibacterialproperties.Beta-lactamantibioticsaretypicallyusedtotreatabroadspectrumofGram-positiveandGram-negativebacteria.Beta-lactamasesproducedbyGram-negativeorganismsareusuallysecreted,especiallywhenantibioticsarepresentintheenvironment.[1]Contents1Structure2Penicillinase3ResistanceinGram-negativebacteria3.1Extended-spectrumbeta-lactamase(ESBL)3.2Types3.2.1TEMbeta-lactamases(classA)3.2.2SHVbeta-

AmpC β-lactamases, which confer resistance against a broad range of substrates, are less prevalent than ESBL but still a growing issue, ...ThisarticleispartoftheResearchTopicMALDI-TOFMSApplicationforSusceptibilityTestingofMicroorganismsViewall12ArticlesArticlesMiklosFuziSemmelweisUniversity,HungaryFilipaGrossoFacultyofPharmacy,UniversityofPorto,PortugalArmandPaauwNetherlandsOrganisationforAppliedScientificResearch,NetherlandsTheeditorandreviewers'affiliationsarethelatestprovidedontheirLoopresearchprofilesandmaynotreflecttheirsituationatthetimeofreview.AbstractIntroductionMaterialsandMethodsResultsDiscussionAuthorContributionsFundingConflictofInterestStatementAcknowledgmentsSupplementaryMaterialReferencesSuggestaResearchTopic>DownloadArticleDownloadPDFReadCubeEPUBXML(NLM)SupplementaryMaterialExportcitationEndNoteReferenceManagerSimpleTEXTfileBibTextotalviewsViewArticleImpactSuggestaResearchTopic>SHAREONOpenSupplementalDataMETHODSarticleFront.Microbiol.,24January2019|https://doi.org/10.3389/fmicb.2019.00013RapidDetectionofExtended-Spectrumβ-Lactamases(ESBL)andAmpCβ-LactamasesinEnterobacterales:DevelopmentofaScreeningPanelUsingtheMALDI-TOFMS-BasedDirect-on-TargetMicrodropletGrowthAssayCarlosL.Correa-Martínez1†,EvgenyA.Idelevich1†,KatrinSparbier2,MarkusKostrzewa2andKarstenBecker1*1InstituteofMedicalMicrobiology,UniversityHospitalMünster,Münster,Germany2BrukerDaltonikGmbH,Bremen,GermanyIntroduction:Antibioticresistantbacteriaareagrowingconcernworldwide.Extended-spectrumβ-lactamases(ESBL)representthemostcommonresistancemechanismofGram-negativebacteriaagainstβ-lactams,underliningtheneedforadequatediagnosticmethodsthatprovidereliableinformationintheshortesttimepossible.AmpC,alessprevalentbutincreasinglyrelevantclassofβ-lactamases,poseanadditionalchallengeastheirdetectioniscomplex.Here,wepresentanESBLandAmpCscreeningpanelemployingtheMALDI-TOFMS-baseddirect-on-targetmicrodropletgrowthassay(DOT-MGA).MaterialsandMethods:FourreferencestrainsrecommendedbytheEuropeanCommitteeonAnt

NCBISkiptomaincontentSkiptonavigationResourcesHowToAboutNCBIAccesskeysMyNCBISignintoNCBISignOutTryoutPMCLabsandtelluswhatyouthink.LearnMore.JournalListClinMicrobiolRevv.22(1);2009JanPMC2620637ClinMicrobiolRev.2009Jan;22(1):161–182.doi: 10.1128/CMR.00036-08PMCID:PMC2620637PMID:19136439AmpCβ-LactamasesGeorgeA.Jacoby*GeorgeA.JacobyLaheyClinic,Burlington,MassachusettsFindarticlesbyGeorgeA.JacobyAuthorinformationCopyrightandLicenseinformationDisclaimerLaheyClinic,Burlington,Massachusetts*Mailingaddress:LaheyClinic,41MallRoad,Burlington,MA01805.Phone:(781)744-2928.Fax:(781)744-5486.E-mail:[email protected]©2009,AmericanSocietyforMicrobiologyThisarticlehasbeencitedbyotherarticlesinPMC.Summary:AmpCβ-lactamasesareclinicallyimportantcephalosporinasesencodedonthechromosomesofmanyoftheEnterobacteriaceaeandafewotherorganisms,wheretheymediateresistancetocephalothin,cefazolin,cefoxitin,mostpenicillins,andβ-lactamaseinhibitor-β-lactamcombinations.Inmanybacteria,AmpCenzymesareinducibleandcanbeexpressedathighlevelsbymutation.Overexpressionconfersresistancetobroad-spectrumcephalosporinsincludingcefotaxime,ceftazidime,andceftriaxoneandisaproblemespeciallyininfectionsduetoEnterobacteraerogenesandEnterobactercloacae,whereanisolateinitiallysusceptibletotheseagentsmaybecomeresistantupontherapy.TransmissibleplasmidshaveacquiredgenesforAmpCenzymes,whichconsequentlycannowappearinbacterialackingorpoorlyexpressingachromosomalblaAmpCgene,suchasEscherichiacoli,Klebsiellapneumoniae,andProteusmirabilis.Resistanceduetoplasmid-mediatedAmpCenzymesislesscommonthanextended-spectrumβ-lactamaseproductioninmostpartsoftheworldbutmaybebothhardertodetectandbroaderinspectrum.AmpCenzymesencodedbybothchromosomalandplasmidgenesarealsoevolvingtohydrolyzebroad-spectrumcephalosporinsmoreefficiently.TechniquestoidentifyAmpCβ-lactamase-producingisolatesareavailablebutarestillevolvingandarenotyetoptimizedfortheclinicallaboratory,whichprobablynowunderestimatesthisresistancemechanism.Carbapenemscanusuall

Majority of ESBLs and AmpC beta-lactamases producing GNB were isolated from urine specimens 47.5% (116/338). Ampicillin (75.4%), amoxicillin ...BrowseSubjectAreas?ClickthroughthePLOStaxonomytofindarticlesinyourfield.FormoreinformationaboutPLOSSubjectAreas,clickhere.ArticleAuthorsMetricsCommentsMediaCoverageReaderComments(0)FiguresFiguresAbstractBackgroundExtendedspectrumBeta-lactamases(ESBLs)andAmpCbeta-lactamases(AmpC)arethecommonenzymesproducedbygramnegativebacilli,whicharetheirmainmechanismsofresistancetoallgenerationsofcephalosporins.Hence,thisstudyaimedtodeterminethemagnitudeofESBLsandAmpCproducinggramnegativebacilli(GNB)isolatedfromclinicalspecimensatInternationalclinicalLaboratoriesinAddisAbaba,Ethiopia.MethodsAcrosssectionalstudywasconductedfromJanuarytoMay2018.Fromdifferentclinicalspecimens,338GNBwereisolatedandcharacterized.Bacterialspeciesidentification,antimicrobialsusceptibilitytestingandscreeningforESBLsandAmpCproductionwereperformedusingPhoenixautomatedsystem(BDphoenix100).ESBLsproductionwasconfirmedusingacombinationdiscmethod.AllCefoxitinresistantandconfirmedESBLsproducingGNBwereconfirmedforAmpCbeta-lactamasesproductionbyAmpCconfirmatoryNeo-Sensitabsdiscs(ROSCOtablet).DatawereanalyzedusingSPSSversion20software.ResultsE.coli66.0%(224/338)followedbyK.pneumoniae12.1%(41/338)wereGNBmostfrequentlyisolated.TheoverallmagnitudeofESBLsproducingGNBwas38.8%(131/338)andtheextentofAmpCbeta-lactamaseproducingGNBwas2.4%(8/338).MajorityofESBLsandAmpCbeta-lactamasesproducingGNBwereisolatedfromurinespecimens47.5%(116/338).Ampicillin(75.4%),amoxicillinwithclavulanicacid(64.0%)andsulfamethoxazole-trimethoprim(55.6%)weremosttheantibioticstowhichresistancewasmostcommonlyfound.Themultidrugresistance(MDR)levelofGNBwas74.0%(250/338).OfESBLsandAmpCbeta-lactamasesproducingGNB,99.3%wereMDR(p<0.05).ConclusionThehighmagnitudeofESBLsandAmpCbeta-lactamasesproducingGNBcallstheneedsofstronginterventiontominimizefurtheroccurrenceandspreadofsuchGNB.Moreimportantly,theMDRlevelwashighwhi