NCBISkiptomaincontentSkiptonavigationResourcesHowToAboutNCBIAccesskeysMyNCBISignintoNCBISignOutTryoutPMCLabsandtelluswhatyouthink.LearnMore.JournalListJLabPhysiciansv.6(1);Jan-Jun2014PMC3969634JLabPhysicians.2014Jan-Jun;6(1):1–6.doi: 10.4103/0974-2727.129082PMCID:PMC3969634PMID:24696552DetectionofAmpCβLactamasesinGram-negativeBacteriaGunjanGupta,VibhorTak,andPurvaMathurGunjanGuptaDepartmentofLaboratoryMedicine,JaiPrakashNarainApexTraumaCentre,AllIndiaInstituteofMedicalSciences,NewDelhi,IndiaFindarticlesbyGunjanGuptaVibhorTakDepartmentofLaboratoryMedicine,JaiPrakashNarainApexTraumaCentre,AllIndiaInstituteofMedicalSciences,NewDelhi,IndiaFindarticlesbyVibhorTakPurvaMathurDepartmentofLaboratoryMedicine,JaiPrakashNarainApexTraumaCentre,AllIndiaInstituteofMedicalSciences,NewDelhi,IndiaFindarticlesbyPurvaMathurAuthorinformationCopyrightandLicenseinformationDisclaimerDepartmentofLaboratoryMedicine,JaiPrakashNarainApexTraumaCentre,AllIndiaInstituteofMedicalSciences,NewDelhi,IndiaAddressforcorrespondence:Dr.PurvaMathur,E-mail:ni.oc.oohay@ruhtamavrupCopyright:©JournalofLaboratoryPhysiciansThisisanopen-accessarticledistributedunderthetermsoftheCreativeCommonsAttribution-Noncommercial-ShareAlike3.0Unported,whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperlycited.ThisarticlehasbeencitedbyotherarticlesinPMC.AmpCβ-lactamasesareclinicallyimportantcephalosporinasesencodedonthechromosomesofmanyEnterobacteriaceaeandafewotherorganisms,wheretheymediateresistancetocephalothin,cefazolin,cefoxitin,mostpenicillins,andβ-lactamaseinhibitor/β-lactamcombinations.TheincreaseinantibioticresistanceamongGram-negativebacteriaisanotableexampleofhowbacteriacanprocure,maintainandexpressnewgeneticinformationthatcanconferresistancetooneorseveralantibiotics.Detectionoforganismsproducingtheseenzymescanbedifficult,becausetheirpresencedoesnotalwaysproducearesistantphenotypeonconventionaldiscdiffusionorautomatedsusceptibilitytestingmethods.Theseenzymesareoftena