All three isolates carried the blaNDM-1 gene and co-expressed the extended-spectrum β-lactamases CTX-M-15 and SHV-5, as well as the ...SkiptoMainContentAdvertisementSearchMenuAccountMenuMenuSignInRegisterNavbarSearchFilterThisissueAllJournalofAntimicrobialChemotherapyAllBSACJournalsAllJournalsMobileMicrositeSearchTermSearchSignInRegisterIssuesMoreContentAdvancearticlesEditor'sChoiceSupplementsSubmitAuthorGuidelinesSubmissionSiteOpenAccessPurchaseAlertsAboutAbouttheJournalofAntimicrobialChemotherapyEditorialBoardAdvertisingandCorporateServicesJournalsCareerNetworkSelf-ArchivingPolicyDispatchDatesContactBSACIssuesMoreContentAdvancearticlesEditor'sChoiceSupplementsSubmitAuthorGuidelinesSubmissionSiteOpenAccessPurchaseAlertsAboutAbouttheJournalofAntimicrobialChemotherapyEditorialBoardAdvertisingandCorporateServicesJournalsCareerNetworkSelf-ArchivingPolicyDispatchDatesContactBSACClosesearchfilterThisissueAllJournalofAntimicrobialChemotherapyAllBSACJournalsAllJournalssearchinputSearchAdvancedSearchSearchMenuSkipNavDestinationArticleNavigationClosemobilesearchnavigationArticleNavigationVolume66Issue12December2011ArticleContentsAbstractIntroductionMaterialsandmethodsResultsanddiscussionFundingTransparencydeclarationsReferencesArticleNavigationEmergenceofNDM-1-producingKlebsiellapneumoniaeinMoroccoLaurentPoirel,LaurentPoirel*1ServicedeBactériologie-Virologie,INSERMU914‘EmergingResistancetoAntibiotics’,HôpitaldeBicêtre,AssistancePublique/HôpitauxdeParis,FacultédeMédecineParisSud,K.-Bicêtre,France*Correspondingauthor.ServicedeBactériologie-Virologie,HôpitaldeBicêtre,78rueduGénéralLeclerc,94275K.-Bicêtrecedex,France.Tel:+33-1-45-21-36-24;Fax:+33-1-45-21-63-40;E-mail:[email protected]:OxfordAcademicPubMedGoogleScholarAminaBenouda,AminaBenouda2DepartmentofMicrobiology,FacultyofMedicineV,CheikhZaidHospital,Rabat,MoroccoSearchforotherworksbythisauthoron:OxfordAcademicPubMedGoogleScholarConstantinHays,ConstantinHays1ServicedeBa

To the Editor: The novel metallo-β-lactamase named New Delhi metallo-β-lactamase (NDM-1) was identified from Klebsiella pneumoniae and Escherichia coli ...NCBISkiptomaincontentSkiptonavigationResourcesHowToAboutNCBIAccesskeysMyNCBISignintoNCBISignOutTryoutPMCLabsandtelluswhatyouthink.LearnMore.JournalListEmergInfectDisv.18(3);2012MarPMC3309569EmergInfectDis.2012Mar;18(3):532–534.doi: 10.3201/eid1803.1103890PMCID:PMC3309569PMID:22377049NDM-1–producingKlebsiellapneumoniae,CroatiaAnnaritaMazzariol,ZrinkaBošnjak,PieroBallarini,AnaBudimir,BrankaBedenić,SmiljaKalenić,andGiuseppeCornagliaAuthorinformationArticlenotesCopyrightandLicenseinformationDisclaimerUniversityofVerona,Verona,Italy(A.Mazzariol,P.Ballarini,G.Cornaglia);UniversityofZagreb,Zagreb,Croatia(Z.Bošnjak,A.Budimir,B.Bedenić,S.Kalenić)Correspondingauthor.Addressforcorrespondence:AnnaritaMazzariol,DepartmentofPathologyandDiagnostics,UniversityofVerona,StradaLeGrazie,837134Verona,Italy;email:[email protected]:NDMmetallo-beta-lactamase,carbapenemresistance,Klebsiellapneumoniae,bacteria,CroatiaCopyrightnoticeThisarticlehasbeencitedbyotherarticlesinPMC.TotheEditor:Thenovelmetallo-β-lactamasenamedNewDelhimetallo-β-lactamase(NDM-1)wasidentifiedfromKlebsiellapneumoniaeandEscherichiacoliisolatesinSwedenfromapatientpreviouslyhospitalizedinIndia(1).NDM-1isspreadingrapidlyworldwidetononclonallyrelatedisolates,manyofwhicharedirectlyorindirectlytrackedtotheIndiansubcontinent(2).Acarbapenem-resistantK.pneumoniaestrain,KLZA,wasisolatedinMay2009fromthecultureofabloodsamplefromofa40-year-oldmanonthedayafterhisadmissiontoasurgicalintensivecareunitoftheClinicalHospitalCenterinZagreb,Croatia.Thepatienthadbeentransferredafter5daysofhospitalizationinBosniaandHerzegovinafollowingacaraccident.Theclinicalhistorymentionedantimicrobialdrugtreatmentthatdidnotincludecarbapenems(gentamicin,metronidazole,andceftriaxone)andnolinktotheIndiansubcontinent.AntimicrobialdrugsusceptibilitytestingwasperformedbyVitek2(bioMérieux,Marcy

In conclusion, NDM-1-resistant bacteria exhibit multidrug resistance and spread to a certain extent in the hospital via efficient plasmid ...ThisarticleispartoftheResearchTopicAntimicrobialResistanceAsaGlobalPublicHealthProblem:HowCanWeAddressIt?Viewall50ArticlesArticlesIlanaL.CamargoInstituteofPhysicsofSãoCarlos,UniversityofSaoPaulo,BrazilAndresF.Opazo-CapurroUniversityofConcepcion,ChileZhiRuanZhejiangUniversity,ChinaTheeditorandreviewers'affiliationsarethelatestprovidedontheirLoopresearchprofilesandmaynotreflecttheirsituationatthetimeofreview.AbstractIntroductionMaterialsandMethodsResultsDiscussionConclusionDataAvailabilityStatementEthicsStatementAuthorContributionsFundingConflictofInterestSupplementaryMaterialFootnotesReferencesSuggestaResearchTopic>DownloadArticleDownloadPDFReadCubeEPUBXML(NLM)SupplementaryMaterialExportcitationEndNoteReferenceManagerSimpleTEXTfileBibTextotalviewsViewArticleImpactSuggestaResearchTopic>SHAREONOpenSupplementalDataORIGINALRESEARCHarticleFront.Microbiol.,30April2020|https://doi.org/10.3389/fmicb.2020.00700ResistanceofKlebsiellapneumoniaeStrainsCarryingblaNDM–1GeneandtheGeneticEnvironmentofblaNDM–1TianxinXiang1†,ChuanhuiChen2†,JiangxiongWen3,YangLiu1,QiZhang1,NaCheng3,XiaopingWu3andWeiZhang2*1DepartmentofHospitalInfectionControl,TheFirstAffiliatedHospitalofNanchangUniversity,Nanchang,China2DepartmentofRespiratoryandCriticalCare,TheFirstAffiliatedHospitalofNanchangUniversity,Nanchang,China3DepartmentofInfectiousDisease,TheFirstAffiliatedHospitalofNanchangUniversity,Nanchang,ChinaObjective:Regionaldisseminationisthemajorcauseofthewidespreadprevalenceofaplasmid-encodingNDM-1enzyme.Weinvestigatedthedrugresistance,jointefficiency,andgeneenvironmentofaKlebsiellapneumoniaestraincarryingblaNDM–1gene.MaterialsandMethods:Carbapenem-non-susceptiblestrainswereanalyzedusingtheVITEK2Compact.StrainscarryingblaNDM–1wereidentifiedusingpolymerasechainreactionandsequencing.Antimicrobialsusceptibilitytestingandplasmidconjugationexperimentswerethenconduc

Outbreak of Multidrug Resistant NDM-1-Producing Klebsiella pneumoniae from a Neonatal Unit in Shandong Province, China. Yan Jin,.BrowseSubjectAreas?ClickthroughthePLOStaxonomytofindarticlesinyourfield.FormoreinformationaboutPLOSSubjectAreas,clickhere.ArticleAuthorsMetricsCommentsMediaCoverageReaderComments(0)FiguresFiguresAbstractDespiteworldwidedisseminationofNewDelhimetallo-β-lactamase1(blaNDM-1),outbreaksremainuncommoninChina.Inthisstudy,wedescribethecharacteristicsoftheoutbreak-relatedblaNDM-1-producingK.pneumoniaisolatesinaneonatalunitinShandongprovince,China.Werecovered21non-repetitivecarbapenem-resistantK.pneumoniaeisolateswithapositivelymodifiedHodgetest(MHT)orEDTAsynergistictestfrompatientsandenvironmentalsamplesinShandongprovincialhospital.Pulsed-fieldgelelectrophoresis(PFGE)andmultilocussequencetyping(MLST)datashowK.pneumoniaeisolatesfrom19patientswereclonallyrelatedandbelongtotheclonalgroupsST20andST17.Wenotetwooutbreaks,thefirstcausedbyST20duringAugust2012involvingfourpatients,andthesecondcausedbyST20andST17duringJanuary2012andSeptember2013involvingfourteenpatients.Wefoundthebedrailingofonepatientwasthesourceoftheoutbreak.WeverifiedthepresenceoftheblaNDM-1genein21K.pneumoniaeisolates.ThegenesblaCTX-M-15,blaCTX-M-14,blaDHA-1,blaTEM-1andClassIintegronwerealsopresentin18(85.7%),3(14.3%),18(85.7%),19(90.5%)and19(90.5%)isolates,respectively.WealsofoundanisolatewithbothblaNDM-1andblaIMP-4.Alloftheisolatesexhibitedamultidrug-resistancephenotype.Theβ-lactamresistanceof20isolateswastransferableviaconjugation.Inaddition,weshowtheresistanceof21K.pneumoniaeisolatestocarbapenemisnotrelatedtolackofouter-membraneproteinsOmpK35andOmpK36noroverpressionofeffluxpumps.ThisstudyprovidesthefirstreportconfirmingblaNDM-1-producingK.pneumoniaeST20andST17wereassociatedwithoutbreak.Earlydetectionofresistancegenesisaneffectivestrategyinpreventingandcontrollinginfectionbylimitingthedisseminationoftheseorganisms.Citation:JinY,ShaoC,LiJ,FanH,BaiY,WangY(2015)OutbreakofMultidrugResista

The emergence and rapid spread of New Delhi Metallo-beta-lactamase-1 (NDM-1)-producing Klebsiella pneumoniae strains has caused a great ...SkiptomaincontentThankyouforvisitingnature.com.YouareusingabrowserversionwithlimitedsupportforCSS.Toobtainthebestexperience,werecommendyouuseamoreuptodatebrowser(orturnoffcompatibilitymodeinInternetExplorer).Inthemeantime,toensurecontinuedsupport,wearedisplayingthesitewithoutstylesandJavaScript.AdvertisementnaturescientificreportsarticlesarticleGenomicandtranscriptomicanalysisofNDM-1KlebsiellapneumoniaeinspaceflightrevealmechanismsunderlyingenvironmentaladaptabilityDownloadPDFSubjectsBacterialgenomicsPathogensAbstractTheemergenceandrapidspreadofNewDelhiMetallo-beta-lactamase-1(NDM-1)-producingKlebsiellapneumoniaestrainshascausedagreatconcernworldwide.Tobetterunderstandthemechanismsunderlyingenvironmentaladaptationofthosehighlydrug-resistantK.pneumoniaestrains,wetookadvantageoftheChina'sShenzhou10spacecraftmissiontoconductcomparativegenomicandtranscriptomicanalysisofaNDM-1K.pneumoniaestrain(ATCCBAA-2146)beingcultivatedunderdifferentconditions.Thesampleswererecoveredfromsemisolidmediumplacedontheground(Dstrain),insimulatedspacecondition(Mstrain),orinShenzhou10spacecraft(Tstrain)foranalysis.Ourdatarevealedmultiplevariationsunderlyingpathogenadaptationintodifferentenvironmentsintermsofchangesinmorphology,H2O2toleranceandbiofilmformationability,genomicstabilityandregulationofmetabolicpathways.Additionally,wefoundafewnon-codingRNAstobedifferentiallyregulated.Theresultsarehelpfulforbetterunderstandingtheadaptivemechanismsofdrug-resistantbacterialpathogens.DownloadPDFIntroductionKlebsiellapneumoniaeisahighlyimportantbacterialpathogenthatcausesawiderangeofcommunity-andhospital-acquiredinfections,suchaspneumonia,urinarytractinfections,intra-abdominalinfectionsandrespiratorytractinfections1,2.Theemergenceandrapidspreadofcarbapenem-resistantK.pneumoniaeisolatessuchastheNewDelhiMetallo-beta-lactamase-1(NDM-1)-producingstrainshascausedgreatcon

A carbapenem-resistant Klebsiella pneumoniae strain, Kp5196, was responsible for an uncomplicated cystitis in a patient living at home and ...RequestUsernameCan'tsignin?Forgotyourusername?EnteryouremailaddressbelowandwewillsendyouyourusernameEmail*CloseIftheaddressmatchesanexistingaccountyouwillreceiveanemailwithinstructionstoretrieveyourusernameCreateanewaccountEmailReturninguserAmericanSocietyforMicrobiology("ASM")iscommittedtomaintainingyourconfidenceandtrustwithrespecttotheinformationwecollectfromyouonwebsitesownedandoperatedbyASM("ASMWebSites")andothersources.ThisPrivacyPolicysetsforththeinformationwecollectaboutyou,howweusethisinformationandthechoicesyouhaveabouthowweusesuchinformation.ACCEPT thecookiepolicyand& CONTINUEFINDOUTMOREabouttheprivacypolicyChangePasswordOldPasswordNewPasswordTooShortWeakMediumStrongVeryStrongTooLongPasswordChangedSuccessfullyYourpasswordhasbeenchangedLoginRegisterEmail*Password*Forgotpassword?ResetithereKeepmeloggedinFieldswith*aremandatoryDon'thaveanaccount?CreateonehereEmail*Fieldswith*aremandatoryAlreadyhaveanaccount?LoginhereCan'tsignin?Forgotyourpassword?EnteryouremailaddressbelowandwewillsendyoutheresetinstructionsEmail*Retypethecodefromthepicture: CancelIftheaddressmatchesanexistingaccountyouwillreceiveanemailwithinstructionstoresetyourpassword.CloseVerifyPhoneEntertheverificationcodeCancelCongrats!YourPhonehasbeenverifiedcloseCLOSEEnterwords/phrases/DOI/ISBN/keywords/authors/etcSearchSearchAdvancedsearchSuggestedTerms:authorinformationmigrationinformationpublicationfeesCOVID-19collectionasmjournalscollections

The emergence of carbapenem-resistant Klebsiella pneumoniae (CR-KP) has become a significant problem worldwide and also being a major threat ...SkiptomaincontentAdvertisementSearchallBMCarticlesSearchDownloadPDFResearcharticleOpenAccessPublished:14November2018CharacteristicsofNDM-1-producingKlebsiellapneumoniaeST234andST1412isolatesspreadinaneonatalunitXuHuang1,2 na1,XiangjunCheng1,2 na1,PengfeiSun1,2,ChenjieTang1,2,FangNi1,2&GenyanLiu ORCID:orcid.org/0000-0002-1004-25171,2 BMCMicrobiologyvolume 18,Article number: 186(2018)Citethisarticle1944Accesses8Citations1AltmetricMetricsdetailsAbstractBackgroundTheemergenceofcarbapenem-resistantKlebsiellapneumoniae(CR-KP)hasbecomeasignificantproblemworldwideandalsobeingamajorthreattochildrenandnewborns.HerewereportanoutbreakofNDM-1-producingK.pneumoniaeinaneonatalunit.ResultsSixCR-KPstrains,isolatedfromneonateswithsymptomsofinfection,wereidentifiedusingaVITEK-2compactsystem,andtheclinicaldatawereretrievedfromtheelectroniccaserecords.InvitrosusceptibilitytestingwithbrothdilutionmethodshowedthatallsixK.pneumoniaeisolateswereresistanttocarbapenemsandsusceptibletocolistin,aminoglycosides,fluoroquinolonesandtigecycline.Basedonthepolymerasechainreactionresults,eachisolatewasfoundtobeblaNDM-1genepositive.Clonalrelationshipswereanalysedusingpulsed-fieldgelelectrophoresis(PFGE)andmultilocussequencetyping(MLST)andshowedthattwodifferentPFGEpatternswereformed,whichbelongedtosequencetypesST234andST1412.PlasmidscarryingblaNDM-1weresuccessfullytransferredfromfourofthesixisolatestoanEscherichiacolirecipientthroughconjugativeassays.S1-PFGEandSouthernblothybridizationshowedthatfourNDM-1-producingK.pneumoniaewereclonalandcarriedblaNDM-1onthesameplasmid.Theoutbreakwaseffectivelycontrolledbyreducingthepotentialinfectionsources.Allthepatientsweresuccessfullytreatedandrecoveredafterreceivinganincreaseddoseofcarbapenems.Althoughthesourceofthisoutbreakwasnotclear,comprehensivemeasureswerecarriedoutandtheoutbreakwaseffectivelycontrolled.ConclusionsST23

NCBISkiptomaincontentSkiptonavigationResourcesHowToAboutNCBIAccesskeysMyNCBISignintoNCBISignOutTryoutPMCLabsandtelluswhatyouthink.LearnMore.JournalListFrontCellInfectMicrobiolv.8;2018PMC5786545FrontCellInfectMicrobiol.2018;8:4.Publishedonline2018Jan22.doi: 10.3389/fcimb.2018.00004PMCID:PMC5786545PMID:29404282Colonization,Infection,andtheAccessoryGenomeofKlebsiellapneumoniaeRebekahM.MartinandMichaelA.Bachman*AuthorinformationArticlenotesCopyrightandLicenseinformationDisclaimerDepartmentofPathology,MichiganMedicine,UniversityofMichigan,AnnArbor,MI,UnitedStatesEditedby:KathrynElizabethHolt,UniversityofMelbourne,AustraliaReviewedby:HaijianZhou,NationalInstituteforCommunicableDiseaseControlandPrevention(ChinaCDC),China;Hong-YuOu,ShanghaiJiaoTongUniversity,China;SylvainBrisse,InstitutPasteur,France*Correspondence:MichaelA.Bachmanude.hcimu.dem@hcabekimReceived2017Sep30;Accepted2018Jan5.Copyright©2018MartinandBachman.Thisisanopen-accessarticledistributedunderthetermsoftheCreativeCommonsAttributionLicense(CCBY).Theuse,distributionorreproductioninotherforumsispermitted,providedtheoriginalauthor(s)orlicensorarecreditedandthattheoriginalpublicationinthisjournaliscited,inaccordancewithacceptedacademicpractice.Nouse,distributionorreproductionispermittedwhichdoesnotcomplywiththeseterms.ThisarticlehasbeencitedbyotherarticlesinPMC.KlebsiellapneumoniaeisaGram-negativepathogenthathasalargeaccessorygenomeofplasmidsandchromosomalgeneloci.ThisaccessorygenomedividesK.pneumoniaestrainsintoopportunistic,hypervirulent,andmultidrug-resistantgroupsandseparatesK.pneumoniaefromtwocloselyrelatedspecies,KlebsiellavariicolaandKlebsiellaquasipneumoniae.SomestrainsofK.pneumoniaeactasopportunisticpathogens,infectingcriticallyillandimmunocompromisedpatients.TheseK.pneumoniaeareacommoncauseofhealth-careassociatedinfectionsincludingpneumonia,urinarytractinfections(UTIs),andbloodstreaminfections.K.variicolaandK.quasipneumoniaeareoftenclinicallyindistinguishablefromopportunisticK.pneumoniae.OtherstrainsofK

Although first and most often detected in Klebsiella pneumoniae and Escherichia coli, the NDM-1 gene can spread rapidly and has been found in ...SkiptoMainContentAdvertisementSearchMenuAccountMenuMenuSignInRegisterNavbarSearchFilterThisissueAllJournalofAntimicrobialChemotherapyAllBSACJournalsAllJournalsMobileMicrositeSearchTermSearchSignInRegisterIssuesMoreContentAdvancearticlesEditor'sChoiceSupplementsSubmitAuthorGuidelinesSubmissionSiteOpenAccessPurchaseAlertsAboutAbouttheJournalofAntimicrobialChemotherapyEditorialBoardAdvertisingandCorporateServicesJournalsCareerNetworkSelf-ArchivingPolicyDispatchDatesContactBSACIssuesMoreContentAdvancearticlesEditor'sChoiceSupplementsSubmitAuthorGuidelinesSubmissionSiteOpenAccessPurchaseAlertsAboutAbouttheJournalofAntimicrobialChemotherapyEditorialBoardAdvertisingandCorporateServicesJournalsCareerNetworkSelf-ArchivingPolicyDispatchDatesContactBSACClosesearchfilterThisissueAllJournalofAntimicrobialChemotherapyAllBSACJournalsAllJournalssearchinputSearchAdvancedSearchSearchMenuSkipNavDestinationArticleNavigationClosemobilesearchnavigationArticleNavigationVolume68Issue5May2013ArticleContentsFundingTransparencydeclarationsAcknowledgementsReferencesArticleNavigationEmergenceofKlebsiellapneumoniaeproducingNDM-1carbapenemaseinSaintPetersburg,RussiaE.P.Barantsevich,E.P.Barantsevich*1AlmazovFederalCentreofHeart,BloodandEndocrinology,SaintPetersburg,Russia2I.P.PavlovStateMedicalUniversity,SaintPetersburg,Russia*Correspondingauthor.Tel:+7-911-7472257;Fax:+7-812-7023700;E-mail:[email protected]:OxfordAcademicPubMedGoogleScholarI.V.Churkina,I.V.Churkina1AlmazovFederalCentreofHeart,BloodandEndocrinology,SaintPetersburg,RussiaSearchforotherworksbythisauthoron:OxfordAcademicPubMedGoogleScholarN.E.Barantsevich,N.E.Barantsevich1AlmazovFederalCentreofHeart,BloodandEndocrinology,SaintPetersburg,RussiaSearchforotherworksbythisauthoron:OxfordAcademicPubMedGoogleScholarJ.Pelkonen,J.Pelkonen3Universityo