New Delhi metallo-beta-lactamase-1 (NDM-1) is a bacterial enzyme that renders the bacteria resistant to a variety of beta-lactam antibiotics.BrasilRevistadaSociedadeBrasileiradeMedicinaTropicalSubmissãodemanuscritosSobreoperiódicoCorpoEditorialInstruçõesaosautoresContatoEspañolEnglishBrasilEspañolEnglishRevistadaSociedadeBrasileiradeMedicinaTropicalSubmissãodemanuscritosSobreoperiódicoCorpoEditorialInstruçõesaosautoresContatosumário«anterioratualseguinte»Texto(EN)Texto(Inglês)PDFDownloadPDF(Inglês)CompartilheCompartilheE-mailFacebookTwitterGoogle+LinkedInRedditStambleUponCiteULikeMendeleySumárioCompartilheE-mailFacebookTwitterGoogle+LinkedInRedditStambleUponCiteULikeMendeleyTexto(EN)Texto(Inglês)PDFDownloadPDF(Inglês)CaseReport•Rev.Soc.Bras.Med.Trop.49(1)•Jan-Feb 2016•https://doi.org/10.1590/0037-8682-0150-2015 copyNewDelhimetallo-beta-lactamase-1-producingAcinetobacterspp.infection:reportofasurvivorFabianaSchuelter-TrevisolProgramadePós-GraduaçãoemCiênciasdaSaúde,UniversidadedoSuldeSantaCatarina,Tubarão,SantaCatarina,Brazil.CentrodePesquisasClínicas,HospitalNossaSenhoradaConceição,Tubarão,SantaCatarina,Brazil.GracianeJacintaSchmittProgramadePós-GraduaçãoemCiênciasdaSaúde,UniversidadedoSuldeSantaCatarina,Tubarão,SantaCatarina,Brazil.JaneMartinsdeAraújoProgramadePós-GraduaçãoemCiênciasdaSaúde,UniversidadedoSuldeSantaCatarina,Tubarão,SantaCatarina,Brazil.LilianeBragadeSouzaComissãodeControledeInfecçãoHospitalar,HospitalNossaSenhoradaConceição,Tubarão,SantaCatarina,Brazil.JulianaGomesNazárioComissãodeControledeInfecçãoHospitalar,HospitalNossaSenhoradaConceição,Tubarão,SantaCatarina,Brazil.RaquelLanduchiJanuárioComissãodeControledeInfecçãoHospitalar,HospitalNossaSenhoradaConceição,Tubarão,SantaCatarina,Brazil.RogérioSobrozadeMelloComissãodeControledeInfecçãoHospitalar,HospitalNossaSenhoradaConceição,Tubarão,SantaCatarina,Brazil.DaissonJoséTrevisolProgramadePós-GraduaçãoemCiênciasdaSaúde,UniversidadedoSuldeSantaCatarina,Tubarão,SantaCatarina,Brazil.CentrodePesquisasClíni

New Delhi metallo-β-lactamase-1 is a bacterial enzyme that renders all current β-lactam antibiotics inactive. Most patients from whom an NDM-1–producing ...NCBISkiptomaincontentSkiptonavigationResourcesHowToAboutNCBIAccesskeysMyNCBISignintoNCBISignOutTryoutPMCLabsandtelluswhatyouthink.LearnMore.JournalListCMAJv.183(1);2011Jan11PMC3017254CMAJ.2011Jan11;183(1):59–64.doi: 10.1503/cmaj.101487PMCID:PMC3017254PMID:21220461NewDelhimetallo-β-lactamase-1inEnterobacteriaceae:emergingresistanceDylanR.Pillai,MDPhD,AllisonMcGeer,MD,andDonaldE.Low,MDAuthorinformationCopyrightandLicenseinformationDisclaimerFromtheDepartmentofMicrobiology(Pillai,McGeerandLow),MountSinaiHospital,Toronto,Ont.;theOntarioAgencyforHealthProtectionandPromotion,Toronto,Ont.;andtheDepartmentofLaboratoryMedicineandPathobiology,UniversityofToronto,Toronto,Ont.Correspondenceto:Dr.DonaldE.Low,ac.no.ianistm@woldCopyright©1995-2011,CanadianMedicalAssociationThisarticlehasbeencitedbyotherarticlesinPMC.Letusconsiderthefollowinghypotheticalscenario.A48-year-oldmanwithahistoryoftype2diabetesmellituswasrecentlyonvacationinCalcutta,India.Threedaysintohisstay,hewasinvolvedinamotorvehicleaccident,forwhichhewasadmittedtothelocalgeneralhospital.Heunderwentopenreductionandinternalfixationofacomminutedfractureofthelefttibia.Hispostoperativecarewasunremarkableexceptforaurinarytractinfection.Hewasgivencefazolinintravenouslyforfivedays.After12daysinhospital,hewasdischargedandwasassessedbyanorthopedicsurgeonuponhisreturntoCanada.Radiographicexaminationshowedlooseningoftheprosthetichardware.Hewasadmittedtohospitalforrevisionofthehardware.IntraoperativeculturesgrewKlebsiellapneumoniaethatshowedresistancetoβ-lactamantibiotics,fluoroquinolones,cephalosporinsandcarbapenems.AmodifiedHodgetesttodetectcarbapenemasegaveapositiveresult.Theisolatewasforwardedtothelocalpublichealthlaboratory,wherethepresenceofthegeneforNewDelhimetallo-β-lactamase-1(NDM-1)wasconfirmedbypolymerasechainreactionandDNAsequencing.Thecarbapenemclassofantimicrobia

NCBISkiptomaincontentSkiptonavigationResourcesHowToAboutNCBIAccesskeysMyNCBISignintoNCBISignOutTryoutPMCLabsandtelluswhatyouthink.LearnMore.JournalListBioinorgChemApplv.2008;2008PMC2422870BioinorgChemAppl.2008;2008:576297.Publishedonline2008Jun4.doi: 10.1155/2008/576297PMCID:PMC2422870PMID:18551183TheMechanismsofCatalysisbyMetalloβ-LactamasesMichaelI.Page*andAdrianaBadarauMichaelI.PageDepartmentofChemicalandBiologicalSciences,UniversityofHuddersfield,HuddersfieldHD13DH,UKFindarticlesbyMichaelI.PageAdrianaBadarauDepartmentofChemicalandBiologicalSciences,UniversityofHuddersfield,HuddersfieldHD13DH,UKFindarticlesbyAdrianaBadarauAuthorinformationArticlenotesCopyrightandLicenseinformationDisclaimerDepartmentofChemicalandBiologicalSciences,UniversityofHuddersfield,HuddersfieldHD13DH,UK*MichaelI.Page:[email protected];Revised2008Jan29;Accepted2008Feb26.Copyright©2008M.I.PageandA.Badarau.ThisisanopenaccessarticledistributedundertheCreativeCommonsAttributionLicense,whichpermitsunrestricteduse,distribution,andreproductioninanymedium,providedtheoriginalworkisproperlycited.ThisarticlehasbeencitedbyotherarticlesinPMC.ClassBβ-lactamasesormetallo-β-lactamases(MBLs)requirezincionstocatalysethehydrolysisofβ-lactamantibioticssuchaspenicillins,cephalosporins,carbapenems,andcephamycins.TherearenoclinicallyusefulinhibitorsagainstMBLswhichareresponsiblefortheresistanceofsomebacteriatoantibiotics.Therearetwometal-ionbindingsitesthathavedifferentzincligandsbuttheexactrolesofthemetal-ionremaincontroversial,anddistinguishingbetweentheirrelativeimportanceiscomplex.Themetal-ioncanactasaLewisacidbyco-ordinationtotheβ-lactamcarbonyloxygentofacilitatenucleophilicattackandstabilisethenegativechargedevelopedonthisoxygeninthetetrahedralintermediateanion.Themetal-ionalsolowersthepKaofthedirectlyco-ordinatedwatermoleculesothatthemetal-boundhydroxideionisabetternucleophilethanwaterandisusedtoattacktheβ-lactamcarbonylcarbon.Anintrinsicpropertyofbinuclearmet

New Delhi metallo-β-lactamase (NDM) had been reported to be the predominant carbapenemase among Escherichia coli in Taiwan.SkiptomaincontentThankyouforvisitingnature.com.YouareusingabrowserversionwithlimitedsupportforCSS.Toobtainthebestexperience,werecommendyouuseamoreuptodatebrowser(orturnoffcompatibilitymodeinInternetExplorer).Inthemeantime,toensurecontinuedsupport,wearedisplayingthesitewithoutstylesandJavaScript.AdvertisementnaturescientificreportsarticlesarticleIncreasingNewDelhimetallo-β-lactamase-positiveEscherichiacoliamongcarbapenemnon-susceptibleEnterobacteriaceaeinTaiwanduring2016to2018DownloadPDFSubjectsBacteriaClinicalmicrobiologyInfectiousdiseasesMicrobialgeneticsAbstractNewDelhimetallo-β-lactamase(NDM)hadbeenreportedtobethepredominantcarbapenemaseamongEscherichiacoliinTaiwan.However,studiesfocusingontheclonalbackgroundandepidemiologyofplasmidscarryingNDMgeneswerelimited.Between2016and2018,allclinicalE.coliandKlebsiellapneumoniaeisolatesthatwerenon-susceptibletoertapenem,meropenem,andimipenemweretestedforcarbapenemase-encodinggenes(CEGs)andantimicrobialsusceptibilities.Moleculartypingwasperformedonallcarbapenemase-producingisolates.Wholegenomesequencing(WGS)wasperformedonallNDM-positiveE.coliisolates.Twenty-three(29.5%)of78carbapenemnon-susceptibleE.coliand108(35.3%)of306carbapenemnon-susceptibleK.pneumoniaeisolatescarriedCEGs.ThemostprevalentCEGsincarbapenemase-producingE.coli(CPEc)wereblaNDM(39.1%)andblaIMP-8(30.4%),whilethatincarbapenemase-producingK.pneumoniaewasKlebsiellapneumoniaecarbapenemase(KPC)(72.2%).Fifteensequencetypeswereidentifiedamong23CPEc,and55.6%ofNDM-positiveE.coliisolatesbelongedtoST410.WGSshowedST410isolateswerehighlyclonalandsimilartothosefromothercountries.AllNDM-5-positiveE.coliisolatescarriedidenticalIncX3plasmidharboringblaNDM-5butnootherantimicrobialresistance(AMR)genes.IneachofthefourNDM-1-positiveE.coliisolates,theblaNDM-1waspresentina∼300kbIncHI2/IncHI2AplasmidwhichcarriedanarrayofAMRgenes.NDMsarethemostprevalentcarbapenem

Since the first New Delhi metallo-beta-lactamase (NDM) report in 2009, NDM has spread globally causing various types of infections.NCBISkiptomaincontentSkiptonavigationResourcesHowToAboutNCBIAccesskeysMyNCBISignintoNCBISignOutTryoutPMCLabsandtelluswhatyouthink.LearnMore.JournalListInfectDrugResistv.8;2015PMC4554481InfectDrugResist.2015;8:297–309.Publishedonline2015Aug24.doi: 10.2147/IDR.S39186PMCID:PMC4554481PMID:26345624ImpactoftheNewDelhimetallo-beta-lactamaseonbeta-lactamantibioticsMonikaTZmarlicka,1MichaelDNailor,2andDavidPNicolau3MonikaTZmarlicka1DepartmentofPharmacy,HartfordHospital,Hartford,CT,USAFindarticlesbyMonikaTZmarlickaMichaelDNailor2SchoolofPharmacy,DepartmentofPharmacyPractice,UniversityofConnecticut,Storrs,CT,USAFindarticlesbyMichaelDNailorDavidPNicolau3CenterforAnti-InfectiveResearchandDevelopment,HartfordHospital,Hartford,CT,USAFindarticlesbyDavidPNicolauAuthorinformationCopyrightandLicenseinformationDisclaimer1DepartmentofPharmacy,HartfordHospital,Hartford,CT,USA2SchoolofPharmacy,DepartmentofPharmacyPractice,UniversityofConnecticut,Storrs,CT,USA3CenterforAnti-InfectiveResearchandDevelopment,HartfordHospital,Hartford,CT,USACorrespondence:DavidPNicolau,CenterforAnti-InfectiveResearchandDevelopment,HartfordHospital,80SeymourStreet,Hartford,CT06102,USA,Tel+18605453941,Fax+18609723992,[email protected]©2015Zmarlickaetal.ThisworkispublishedbyDoveMedicalPressLimited,andlicensedunderCreativeCommonsAttribution–NonCommercial(unported,v3.0)LicenseThefulltermsoftheLicenseareavailableathttp://creativecommons.org/licenses/by-nc/3.0/.Non-commercialusesoftheworkarepermittedwithoutanyfurtherpermissionfromDoveMedicalPressLimited,providedtheworkisproperlyattributed.ThisarticlehasbeencitedbyotherarticlesinPMC.SincethefirstNewDelhimetallo-beta-lactamase(NDM)reportin2009,NDMhasspreadgloballycausingvarioustypesofinfections.NDM-positiveorganismsproduceinvitroresistancephenotypestocarbapenemsandmanyotherantimicrobials.Itisthussurprisingthatthelite

The spread of carbapenem-resistant Enterobacteriaceae (CRE) mediated by New Delhi metallo-β-lactamase (NDM) poses a serious challenge to ...ArticlesMiklosFuziSemmelweisUniversity,HungaryBenjaminA.EvansUniversityofEastAnglia,UnitedKingdomTommasoGianiUniversityofFlorence,ItalyTheeditorandreviewers'affiliationsarethelatestprovidedontheirLoopresearchprofilesandmaynotreflecttheirsituationatthetimeofreview.AbstractIntroductionMaterialsandMethodsResultsandDiscussionConclusionAuthorContributionsFundingConflictofInterestStatementSupplementaryMaterialFootnoteReferencesSuggestaResearchTopic>DownloadArticleDownloadPDFReadCubeEPUBXML(NLM)SupplementaryMaterialExportcitationEndNoteReferenceManagerSimpleTEXTfileBibTextotalviewsViewArticleImpactSuggestaResearchTopic>SHAREONOpenSupplementalDataORIGINALRESEARCHarticleFront.Microbiol.,21February2018|https://doi.org/10.3389/fmicb.2018.00248NovelVariantofNewDelhiMetallo-β-lactamase,NDM-20,inEscherichiacoliZhihaiLiu1,JiyunLi1,XiaomingWang1,DejunLiu1,YuebinKe2,YangWang1andJianzhongShen1*1BeijingKeyLaboratoryofDetectionTechnologyforAnimal-DerivedFoodSafety,CollegeofVeterinaryMedicine,ChinaAgriculturalUniversity,Beijing,China2KeyLaboratoryofGenetics&MolecularMedicineofShenzhen,ShenzhenCenterforDiseaseControlandPrevention,Shenzhen,ChinaThespreadofcarbapenem-resistantEnterobacteriaceae(CRE)mediatedbyNewDelhimetallo-β-lactamase(NDM)posesaseriouschallengetocliniciansandhasbecomeamajorpublichealthconcern.NDMhasbeenevolvingintovariantsthatpossessdifferenthydrolysisactivitytowardantibiotics,soastoaffecttreatmentstrategy.Inaddition,veryfewstudiesonNDMvariantshavefocusedonanimal-derivedbacterialisolates.OurstudyreportsanovelNDMvariant,NDM-20,inanisolateofEscherichiacoliCCD1recoveredfromthefoodanimalswineinChina.TheisolatethatwasassignedtoST1114,exhibitedhighlevelresistancetoallβ-lactamstested,includingaztreonamandcarbapenems.ThegeneofblaNDM-20waslocatedonanIncX3-typeplasmid,surroundedbymultipleinsertionsequences.SequencinganalysisdemonstratedthatblaN

The first New Delhi metallo-beta-lactamase (MBL), NDM-1, was identified in 2008 in an extensively drug-resistant (XDR) Klebsiella pneumoniae ...ArticlesMiklosFuziSemmelweisUniversity,HungaryLiangLiCenterforDevicesandRadiologicalHealth(CDRH),UnitedStatesFilipaGrossoFacultyofPharmacy,UniversityofPorto,PortugalMariagraziaPerilliUniversityofL'Aquila,ItalyTheeditorandreviewers'affiliationsarethelatestprovidedontheirLoopresearchprofilesandmaynotreflecttheirsituationatthetimeofreview.AbstractIntroductionMaterialsandMethodsResultsDiscussionEthicsStatementAuthorContributionsConflictofInterestStatementAcknowledgmentsSupplementaryMaterialReferencesSuggestaResearchTopic>DownloadArticleDownloadPDFReadCubeEPUBXML(NLM)SupplementaryMaterialExportcitationEndNoteReferenceManagerSimpleTEXTfileBibTextotalviewsViewArticleImpactSuggestaResearchTopic>SHAREONOpenSupplementalDataORIGINALRESEARCHarticleFront.Microbiol.,29March2018|https://doi.org/10.3389/fmicb.2018.00571NewDelhiMetallo-Beta-Lactamase-ProducingEnterobacteriaceaeinSouthKoreaBetween2010and2015Eun-JeongYoon1,DaYoungKang2,JiWooYang3,DokyunKim1,HyukminLee1,KwangJunLee3*andSeokHoonJeong1*1CollegeofMedicine,YonseiUniversity,Seoul,SouthKorea2CollegeofHealthScience,SangjiUniversity,Wonju,SouthKorea3KoreaCentersforDiseaseControlandPrevention,KoreaNationalInstituteofHealth,Cheongju,SouthKoreaThisstudywascarriedouttoinvestigatetheepidemiologicaltime-courseofNewDelhimetallo-beta-lactamase-(NDM-)mediatedcarbapenemresistanceinEnterobacteriaceaeinSouthKorea.Atotalof146non-duplicateNDM-producingEnterobacteriaceaerecoveredbetween2010and2015werevoluntarilycollectedfrom33generalhospitalsandconfirmedbyPCR.Thespecieswereidentifiedbysequencesofthe16SrDNA.Antimicrobialsusceptibilitywasdeterminedeitherbythediskdiffusionmethodorbybrothmicrodilution,andthecarbapenemMICsweredeterminedbyagardilution.Then,multilocussequencetypingandPCR-basedreplicontypingwascarriedout.Co-carriedgenesfordrugresistancewereidentifiedbyPCRandsequencing.Theentiregenomesofeight

New Delhi metallo-beta lactamase (NDM) produced by bacterial isolates from the Indian subcontinent are the latest carbapenemases, which ...SkiptomaincontentAdvertisementSearchallBMCarticlesSearchDownloadPDFReviewOpenAccessPublished:27April2017Structure,GeneticsandWorldwideSpreadofNewDelhiMetallo-β-lactamase(NDM):athreattopublichealthAsadU.Khan1,LubnaMaryam1&RaffaeleZarrilli ORCID:orcid.org/0000-0003-4191-90322,3 BMCMicrobiologyvolume 17,Article number: 101(2017)Citethisarticle15kAccesses194Citations59AltmetricMetricsdetailsAbstractBackgroundTheemergenceofcarbapenemaseproducingbacteria,especiallyNewDelhimetallo-β-lactamase(NDM-1)anditsvariants,worldwide,hasraisedamajorpublichealthconcern.NDM-1hydrolyzesawiderangeofβ-lactamantibiotics,includingcarbapenems,whicharethelastresortofantibioticsforthetreatmentofinfectionscausedbyresistantstrainofbacteria.MainbodyInthisreview,wehavediscussedblaNDM-1variants,itsgeneticanalysisincludingtypeofspecificmutation,originofcountryandspreadamongseveraltypeofbacterialspecies.Widemembersofenterobacteriaceae,mostcommonlyEscherichiacoli,Klebsiellapneumoniae,Enterobactercloacae,andgram-negativenon-fermentersPseudomonasspp.andAcinetobacterbaumanniiwerefoundtocarrythesemarkers.Moreover,atleastseventeenvariantsofblaNDM-typegenedifferingintooneortworesiduesofaminoacidsatdistinctpositionshavebeenreportedsofaramongdifferentspeciesofbacteriafromdifferentcountries.Thegeneticandstructuralstudiesofthesevariantsareimportanttounderstandthemechanismofantibiotichydrolysisaswellastodesignnewmoleculeswithinhibitoryactivityagainstantibiotics.ConclusionThisreviewprovidesacomprehensiveviewofstructuraldifferencesamongNDM-1variants,whichareadrivingforcebehindtheirspreadacrosstheglobe.BackgroundAlthoughantibioticsweredevelopedtofightinfections,theemergenceofnewresistantmarkers,especiallyNewDelhi-metallo-beta-lactamases(NDM-1),hamperedthecapabilityofallantibioticsofbetalactamgrouptotreatinfectionscausedbymicroorganismscarryingsuchresistances.Thepossiblereasonfor

Among them, the New Delhi metallo-β-lactamase (NDM) has experienced the fastest and widest geographical spread. While other clinically important MBLs are ...RequestUsernameCan'tsignin?Forgotyourusername?EnteryouremailaddressbelowandwewillsendyouyourusernameEmail*CloseIftheaddressmatchesanexistingaccountyouwillreceiveanemailwithinstructionstoretrieveyourusernameCreateanewaccountEmailReturninguserAmericanSocietyforMicrobiology("ASM")iscommittedtomaintainingyourconfidenceandtrustwithrespecttotheinformationwecollectfromyouonwebsitesownedandoperatedbyASM("ASMWebSites")andothersources.ThisPrivacyPolicysetsforththeinformationwecollectaboutyou,howweusethisinformationandthechoicesyouhaveabouthowweusesuchinformation.ACCEPT thecookiepolicyand& CONTINUEFINDOUTMOREabouttheprivacypolicyChangePasswordOldPasswordNewPasswordTooShortWeakMediumStrongVeryStrongTooLongPasswordChangedSuccessfullyYourpasswordhasbeenchangedLoginRegisterEmail*Password*Forgotpassword?ResetithereKeepmeloggedinFieldswith*aremandatoryDon'thaveanaccount?CreateonehereEmail*Fieldswith*aremandatoryAlreadyhaveanaccount?LoginhereCan'tsignin?Forgotyourpassword?EnteryouremailaddressbelowandwewillsendyoutheresetinstructionsEmail*Retypethecodefromthepicture: CancelIftheaddressmatchesanexistingaccountyouwillreceiveanemailwithinstructionstoresetyourpassword.CloseVerifyPhoneEntertheverificationcodeCancelCongrats!YourPhonehasbeenverifiedcloseCLOSEEnterwords/phrases/DOI/ISBN/keywords/authors/etcSearchSearchAdvancedsearchSuggestedTerms:authorinformationmigrationinformationpublicationfeesCOVID-19collectionasmjournalscollections