The spread of carbapenem-resistant Enterobacteriaceae (CRE) mediated by New Delhi metallo-β-lactamase (NDM) poses a serious challenge to ...ArticlesMiklosFuziSemmelweisUniversity,HungaryBenjaminA.EvansUniversityofEastAnglia,UnitedKingdomTommasoGianiUniversityofFlorence,ItalyTheeditorandreviewers'affiliationsarethelatestprovidedontheirLoopresearchprofilesandmaynotreflecttheirsituationatthetimeofreview.AbstractIntroductionMaterialsandMethodsResultsandDiscussionConclusionAuthorContributionsFundingConflictofInterestStatementSupplementaryMaterialFootnoteReferencesSuggestaResearchTopic>DownloadArticleDownloadPDFReadCubeEPUBXML(NLM)SupplementaryMaterialExportcitationEndNoteReferenceManagerSimpleTEXTfileBibTextotalviewsViewArticleImpactSuggestaResearchTopic>SHAREONOpenSupplementalDataORIGINALRESEARCHarticleFront.Microbiol.,21February2018|https://doi.org/10.3389/fmicb.2018.00248NovelVariantofNewDelhiMetallo-β-lactamase,NDM-20,inEscherichiacoliZhihaiLiu1,JiyunLi1,XiaomingWang1,DejunLiu1,YuebinKe2,YangWang1andJianzhongShen1*1BeijingKeyLaboratoryofDetectionTechnologyforAnimal-DerivedFoodSafety,CollegeofVeterinaryMedicine,ChinaAgriculturalUniversity,Beijing,China2KeyLaboratoryofGenetics&MolecularMedicineofShenzhen,ShenzhenCenterforDiseaseControlandPrevention,Shenzhen,ChinaThespreadofcarbapenem-resistantEnterobacteriaceae(CRE)mediatedbyNewDelhimetallo-β-lactamase(NDM)posesaseriouschallengetocliniciansandhasbecomeamajorpublichealthconcern.NDMhasbeenevolvingintovariantsthatpossessdifferenthydrolysisactivitytowardantibiotics,soastoaffecttreatmentstrategy.Inaddition,veryfewstudiesonNDMvariantshavefocusedonanimal-derivedbacterialisolates.OurstudyreportsanovelNDMvariant,NDM-20,inanisolateofEscherichiacoliCCD1recoveredfromthefoodanimalswineinChina.TheisolatethatwasassignedtoST1114,exhibitedhighlevelresistancetoallβ-lactamstested,includingaztreonamandcarbapenems.ThegeneofblaNDM-20waslocatedonanIncX3-typeplasmid,surroundedbymultipleinsertionsequences.SequencinganalysisdemonstratedthatblaN